Overview

  • Product name
  • Description
    Rabbit polyclonal to AFF4
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rabbit, Non human primates
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human AFF4.

    (Peptide available as ab89994.)

  • Positive control
    • This antibody gave a positive signal in the following mouse tissue lysates: Liver; Hippocampus; and cell lysate U87-MG

Properties

Applications

Our Abpromise guarantee covers the use of ab75866 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 127 kDa (predicted molecular weight: 127 kDa).

Target

  • Relevance
    May play a role in transcriptional regulation. Component of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor b (P-TEFb).
  • Cellular localization
    Nuclear
  • Database links
  • Alternative names
    • AF4/FMR2 family member 4 antibody
    • AF5Q31 antibody
    • Alf4 antibody
    • ALL1 fused gene from chromosome 5q31 antibody
    • CHOPS antibody
    • HSPC092 antibody
    • Laf4 antibody
    • Lymphoid nuclear protein related to AF4 like antibody
    • Major CDK9 elongation factor associated protein antibody
    • MCEF antibody
    • MGC107469 antibody
    • RP23-226J16.7 antibody
    see all

Images

  • All lanes : Anti-AFF4 antibody (ab75866) at 1 µg/ml

    Lane 1 : Liver (Mouse) Tissue Lysate
    Lane 2 : Hippocampus (Mouse) Tissue Lysate
    Lane 3 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Predicted band size : 127 kDa
    Observed band size : 127 kDa
    Additional bands at : 109 kDa,98 kDa. We are unsure as to the identity of these extra bands.

References

ab75866 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab75866.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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