For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
Rabbit polyclonal to AFF4
Mouse, HumanPredicted to work with:
Rabbit, Non human primates
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human AFF4.
(Peptide available as ab89994.)
- This antibody gave a positive signal in the following mouse tissue lysates: Liver; Hippocampus; and cell lysate U87-MG
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 µg/ml. Detects a band of approximately 127 kDa (predicted molecular weight: 127 kDa).
May play a role in transcriptional regulation. Component of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor b (P-TEFb).
- AF4/FMR2 family member 4 antibody
- AF5Q31 antibody
- Alf4 antibody
Western blot - Anti-AFF4 antibody (ab75866)
All lanes :
Anti-AFF4 antibody (ab75866) at 1 µg/mlLane 1 :
Liver (Mouse) Tissue LysateLane 2 :
Hippocampus (Mouse) Tissue LysateLane 3 :
U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.SecondaryAll lanes :
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080
) at 1/5000 dilutionPredicted band size:
127 kDaObserved band size:
127 kDaAdditional bands at:
109 kDa, 98 kDa. We are unsure as to the identity of these extra bands.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"