Overview

  • Product name
    Anti-AFMID antibody
  • Description
    Rabbit polyclonal to AFMID
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: LPFFLFFHGG YWQSGSKDES AFMVHPLTAQ GVAVVIVAYG IAPKGTLDHM VDQVTRSVAF VQKRY, corresponding to internal sequence amino acids 88-152 of Human AFMID.

  • Positive control
    • Human kidney tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121870 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/250.
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Catalyzes the hydrolysis of N-formyl-L-kynurenine to L-kynurenine, the second step in the conversion of tryptophan to nicotinic acid, NAD(H) and NADP(H). Required for elimination of toxic metabolites.
  • Pathway
    Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 2/2.
  • Sequence similarities
    Belongs to the AFMID family.
  • Cellular localization
    Cytoplasm > cytosol. Nucleus. Predominantly cytosolic. Some fraction is nuclear.
  • Information by UniProt
  • Database links
  • Alternative names
    • afmid antibody
    • AFMID_HUMAN antibody
    • KF antibody
    • Kynurenine formamidase antibody
    • Probable arylformamidase antibody
    see all

Images

  • Lane 1 :
    Lanes 2 - 3 : Anti-AFMID antibody (ab121870)

    Lane 1 : Marker
    Lane 2 : HEK293T cell lysate - vector only transfected
    Lane 3 : HEK293T cell lysate - overexpressing AFMID with a C-terminal myc-DDK tag (~3.1kDa)
  • ab121870, at 1/300 dilution, staining AFMID in renal tubules of paraffin-embedded Human kidney tissue by Immunohistochemistry.

References

ab121870 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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