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Rabbit polyclonal to AFMID
antigen sequence: LPFFLFFHGG YWQSGSKDES AFMVHPLTAQ GVAVVIVAYG IAPKGTLDHM VDQVTRSVAF VQKRY, corresponding to internal sequence amino acids 88-152 of Human AFMID.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/100 - 1/250.
||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Catalyzes the hydrolysis of N-formyl-L-kynurenine to L-kynurenine, the second step in the conversion of tryptophan to nicotinic acid, NAD(H) and NADP(H). Required for elimination of toxic metabolites.
Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 2/2.
Belongs to the AFMID family.
Cytoplasm > cytosol. Nucleus. Predominantly cytosolic. Some fraction is nuclear.
Information by UniProt
- afmid antibody
- AFMID_HUMAN antibody
- KF antibody
Western blot - Anti-AFMID antibody (ab121870)
Lanes 2-3 : Anti-AFMID antibody (ab121870)
Lane 1 : Marker
Lane 2 : HEK293T cell lysate - vector only transfected
Lane 3 : HEK293T cell lysate - overexpressing AFMID with a C-terminal myc-DDK tag (~3.1kDa)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AFMID antibody (ab121870)
ab121870, at 1/300 dilution, staining AFMID in renal tubules of paraffin-embedded Human kidney tissue by Immunohistochemistry.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"