Overview

  • Product name
  • Description
    Rabbit polyclonal to Aftiphilin
  • Tested applications
    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: RENNKINRVN ELNSVKEVAL GRSLDNKGDT DGEDQVCVSE ISIVTNRGFS VEKQGLPTLQ QDEFLQSGVQ SK, corresponding to amino acids 262-333 of Human Aftiphilin.

  • Positive control
    • Human smooth muscle tissue; Human cell line U-2 OS
  • General notes


    ab122239 is mono-specific.

Properties

Applications

Our Abpromise guarantee covers the use of ab122239 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    May play a role in membrane trafficking.
  • Domain
    The WXXF motifs mediate binding of accessory proteins to the ear-domain of AP-1, GGAs and AP-2 through hydrophobic interactions. Selective binding to the GAE domains of AP-1 or to the alpha-ear domain of AP-2 is tuned by the acidic context surrounding the motif and the properties of the second residue of the motif itself.
  • Cellular localization
    Cytoplasm. Colocalizes with AP1G1 and clathrin.
  • Information by UniProt
  • Database links
  • Alternative names
    • AFTIN_HUMAN antibody
    • Aftiphilin antibody
    • Aftiphilin protein antibody
    • Aftph antibody
    • MGC33965 antibody
    • Nbla10388 antibody
    see all

Images

  • ab122239, at 1/500 dilution, staining Aftiphilin in paraffin-embedded Human smooth muscle by Immunohistochemistry.
  • ab122239, at 4µg/ml, staining Aftiphilin in Human cell line U-2 OS by Immunofluorescence. Antibody staining is shown in green.

References

ab122239 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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