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Rabbit polyclonal to AGBL4
Predicted to work with:
Recombinant fragment, corresponding to a region within amino acids 158-441 of Human AGBL4 (Uniprot ID: Q5VU57).
A549 and HepG2 whole cell lysates; Human breast carcinoma tissue; A549 cells.
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate) Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 58 kDa.
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/100 - 1/1000.
Metallocarboxypeptidase that mediates deglutamylation of target proteins. Catalyzes the deglutamylation of polyglutamate side chains generated by post-translational polyglutamylation in proteins such as tubulins. Also removes gene-encoded polyglutamates from the carboxy-terminus of target proteins such as MYLK. Acts as a long-chain deglutamylase and specifically shortens long polyglutamate chains, while it is not able to remove the branching point glutamate, a process catalyzed by AGBL5/CCP5.
Belongs to the peptidase M14 family.
Cytoplasm > cytosol.
Information by UniProt
ATP/GTP binding protein like 4 antibody
ATP/GTP-binding protein-like 4 antibody
Western blot - Anti-AGBL4 antibody (ab155314)
Anti-AGBL4 antibody (ab155314) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 58 kDa 7.5% SDS PAGE
Immunocytochemistry/ Immunofluorescence - Anti-AGBL4 antibody (ab155314)
Immunofluorescence analysis of methanol-fixed A549 cells, labeling AGBL4 with ab155314 at 1/500 dilution. Lower image shows cells co-stained with Hoechst 33342.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGBL4 antibody (ab155314)
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue, labeling AGBL4 with ab155314 at 1/500 dilution.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"