Overview

  • Product name
    Anti-AGBL4 antibody
  • Description
    Rabbit polyclonal to AGBL4
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 158-441 of Human AGBL4 (Uniprot ID: Q5VU57).

  • Positive control
    • A549 and HepG2 whole cell lysates; Human breast carcinoma tissue; A549 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab155314 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 58 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Metallocarboxypeptidase that mediates deglutamylation of target proteins. Catalyzes the deglutamylation of polyglutamate side chains generated by post-translational polyglutamylation in proteins such as tubulins. Also removes gene-encoded polyglutamates from the carboxy-terminus of target proteins such as MYLK. Acts as a long-chain deglutamylase and specifically shortens long polyglutamate chains, while it is not able to remove the branching point glutamate, a process catalyzed by AGBL5/CCP5.
  • Sequence similarities
    Belongs to the peptidase M14 family.
  • Cellular localization
    Cytoplasm > cytosol.
  • Information by UniProt
  • Database links
  • Alternative names
    • AGBL4 antibody
    • ATP/GTP binding protein like 4 antibody
    • ATP/GTP-binding protein-like 4 antibody
    • CBPC6_HUMAN antibody
    • CCP6 antibody
    • Cytosolic carboxypeptidase 6 antibody
    • FLJ14442 antibody
    see all

Images

  • Anti-AGBL4 antibody (ab155314) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size: 58 kDa



    7.5% SDS PAGE
  • Immunofluorescence analysis of methanol-fixed A549 cells, labeling AGBL4 with ab155314 at 1/500 dilution. Lower image shows cells co-stained with Hoechst 33342.
  • Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue, labeling AGBL4 with ab155314 at 1/500 dilution.

References

ab155314 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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