The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 93.5 kDa.
FunctionMetallocarboxypeptidase that mediates tubulin deglutamylation. Specifically catalyzes the deglutamylation of the branching point glutamate side chains generated by post-translational glutamylation in proteins such as tubulins. In contrast, it is not able to act as a long-chain deglutamylase that shortens long polyglutamate chains, a process catalyzed by AGTPBP1/ CCP1, AGBL1/CCP4 and AGBL4/CCP6.
Tissue specificityExpressed in brain.
Sequence similaritiesBelongs to the peptidase M14 family.
Cellular localizationCytoplasm > cytosol. Nucleus. Mainly cytoplasmic. Slight accumulation in the nucleus is observed.