Anti-AGBL5 antibody - N-terminal (ab170541)

Overview

  • Product name
    Anti-AGBL5 antibody - N-terminal
    See all AGBL5 primary antibodies
  • Description
    Rabbit polyclonal to AGBL5 - N-terminal
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human AGBL5 aa 106-135 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q8NDL9

  • Positive control
    • K562 cell lysate; Human brain tissue; U 251MG cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99.91% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab170541 is purified through a protein A column, followed by immunogen affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab170541 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/10 - 1/50.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 98 kDa.

Target

  • Function
    Metallocarboxypeptidase that mediates tubulin deglutamylation. Specifically catalyzes the deglutamylation of the branching point glutamate side chains generated by post-translational glutamylation in proteins such as tubulins. In contrast, it is not able to act as a long-chain deglutamylase that shortens long polyglutamate chains, a process catalyzed by AGTPBP1/ CCP1, AGBL1/CCP4 and AGBL4/CCP6.
  • Tissue specificity
    Expressed in brain.
  • Sequence similarities
    Belongs to the peptidase M14 family.
  • Cellular localization
    Cytoplasm > cytosol. Nucleus. Mainly cytoplasmic. Slight accumulation in the nucleus is observed.
  • Information by UniProt
  • Database links
  • Alternative names
    • AGBL5 antibody
    • ATP/GTP binding protein like 5 antibody
    • ATP/GTP-binding protein-like 5 antibody
    • CBPC5_HUMAN antibody
    • CCP5 antibody
    • Cytosolic carboxypeptidase like protein 5 antibody
    • Cytosolic carboxypeptidase-like protein 5 antibody
    • FLJ21839 antibody
    • Hypothetical protein FLJ21839 antibody
    see all

Images

  • Immunofluorescent analysis of U 251MG cells labeling AGBL5 with ab170541 at 1/10 (green). DAPI was used to stain the cell nuclear (blue).

  • Immunohistochemical analysis in formalin fixed, paraffin embedded Human brain tissue labeling AGBL5 with ab170541 at 1/50, followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Anti-AGBL5 antibody - N-terminal (ab170541) at 1/100 dilution + K562 cell lysate at 35 µg

    Secondary
    Anti-rabbit HRP conjugated antibody at 1/10000 dilution
    Developed using the ECL technique

    Predicted band size : 98 kDa

References

ab170541 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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