Overview

  • Product name
  • Description
    Rabbit polyclonal to AGE
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Cow, Human, Monkey
  • Immunogen

    Other Immunogen Type corresponding to AGE. Glycated ribonuclease (RNase) prepared by incubating RNase with glucose at 37C for several months.

  • Positive control
    • Glycated ovalbumin, BSA and ribonuclease.

Applications

Our Abpromise guarantee covers the use of ab176173 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500.

Target

  • Relevance
    The non enzymatic reaction of reducing carbohydrates with lysine side chains and N terminal amino groups of macromolecules (amino acids, proteins, phospholipids and nucleic acids) is called the Maillard reaction or glycation. The latter products of this process, termed advanced glycation end products (AGEs), adversely affect the functional properties of proteins, lipids and DNA. In long lived tissue proteins, these chemical modifications accumulate with age and may contribute to the pathophysiology of ageing and long term complications of diabetes, atherosclerosis and renal failure.
  • Cellular localization
    Cell Membrane and Secreted
  • Alternative names
    • Advanced glycation end products antibody
    • AGEs antibody

Images

  • Western Blot analysis of proteins (500 ng) incubated either with glucose or not. Left panel corresponds to incubations with unpurified antibody 1) PI and 2) Test bleed. Right panel shows incubation with ab176173 at a 1/500 dilution.

References

This product has been referenced in:
  • Shan G  et al. Increase in Blood Glutathione and Erythrocyte Proteins Related to Glutathione Generation, Reduction and Utilization in African-American Old Women with Diabetes. J Sci Technol Environ 5:N/A (0). Read more (PubMed: 26770888) »

See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Sample
Rat Tissue lysate - whole (Left atrium of heart)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
50 µg
Treatment
Methylglyoxal at 100mg/kg/j in drinking water for 8 weeks with or without metformin at 300mg/kg/j for 2 weeks.
Specification
Left atrium of heart
Blocking step
Milk as blocking agent for 1 minute(s) · Concentration: 0.5% · Temperature: 20°C
Username

Ms. Helene Peyret

Verified customer

Submitted Jun 17 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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