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Advanced Glycation End Products (BSA-AGE and HSA-AGE)
ab23722 is suitable for the detection of different AGE products in tissues, tissue extracts and body fluids.
Our Abpromise guarantee covers the use of ab23722 in the following tested applications.
|WB||Use at an assay dependent concentration. Can be blocked with Native Cow AGE-BSA protein (ab129535).|
|ICC/IF||Use a concentration of 5 µg/ml.|
|ELISA||Use a concentration of 1 µg/ml.|
|IHC-Fr||Use at an assay dependent concentration.|
|IHC-P||1/10000. PubMed: 19223295|
This image is courtesy of an Abreview submitted by Josephine Böhme
12% SDS-PAGE gel run using denaturing conditions. Membrane blocked with 5% BSA for 30 minutes at room temperature. Primary antibody diluted in 5% BSA in TBS-T and incubated for 20 hours in a fridge
ab23722 staining AGE in Rat aoratic tissue sections showing the effects of diabetes and Ator on AGE expression by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formalin. Samples were incubated with primary antibody (1/10000) overnight at 4°C. A secondary antibody Fab fragment was used for 1 hour at room temperature.
The arrows indicated the sites of antibody staining. NC=normal controls, DM=streptozotocin-induced diabetic rats.