Overview

  • Product name
  • Description
    Rabbit polyclonal to AGFG1
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Rabbit, Guinea pig, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • Immunogen

    Synthetic peptide, corresponding to a region between residues 512 and 562 of Human AGFG1 (NP_004495.2)

  • Positive control
    • HeLa or 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab86349 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 58 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function
    Required for vesicle docking or fusion during acrosome biogenesis (By similarity). May play a role in RNA trafficking or localization. In case of infection by HIV-1, acts as a cofactor for viral Rev and promotes movement of Rev-responsive element-containing RNAs from the nuclear periphery to the cytoplasm. This step is essential for HIV-1 replication.
  • Tissue specificity
    Ubiquitously expressed.
  • Sequence similarities
    Contains 1 Arf-GAP domain.
  • Domain
    Contains FG repeats. The FG repeat region is required for acting as a cofactor of HIV-1 Rev.
  • Post-translational
    modifications
    O-glycosylated.
  • Cellular localization
    Nucleus. Cytoplasmic vesicle.
  • Information by UniProt
  • Database links
  • Alternative names
    • AGFG1 antibody
    • AGFG1_HUMAN antibody
    • Arf-GAP domain and FG repeats-containing protein 1 antibody
    • ArfGAP with FG repeats 1 antibody
    • AU045498 antibody
    • C130049H11Rik antibody
    • C85612 antibody
    • D730048C23Rik antibody
    • DKFZp686I15205 antibody
    • HIV 1 Rev binding protein antibody
    • HIV-1 Rev-binding protein antibody
    • HRB antibody
    • MGC116375 antibody
    • MGC116938 antibody
    • MGC116940 antibody
    • Nucleoporin like protein RIP antibody
    • Nucleoporin-like protein RIP antibody
    • RAB antibody
    • Rev interacting protein antibody
    • Rev-interacting protein antibody
    • Rev/Rex activation domain-binding protein antibody
    • RIP antibody
    see all

Images

  • All lanes : Anti-AGFG1 antibody (ab86349) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg


    Predicted band size : 58 kDa


    Exposure time : 10 seconds
  • Detection of AGFG1 by Western Blot of Immunprecipitate.
    ab86349 at 1µg/ml staining AGFG1 in HeLa whole cell lysate immunoprecipitated using ab86349 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 3 seconds.

References

This product has been referenced in:
  • Kubota Y  et al. WGA-based lectin affinity gel electrophoresis: A novel method for the detection of O-GlcNAc-modified proteins. PLoS One 12:e0180714 (2017). Human . Read more (PubMed: 28686627) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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