Specificityab52141 is specific for keratan sulfate glycosaminoglycan chains. It doesn´t react with the core protein or the chondroitin sulfate chain of the aggrecan. Preliminary results, based on western blot analysis of keratan sulfate from different sources, indicate that ab52141 is specific for keratan sulfate from articular cartilage and shows only minimal if any cross-reactivity with keratan sulfate from intervertebral disc.
Primary antibody notesIn addition to the stated applications, ab52141 is well suited for detecting keratan sulfate fragments released during human inflammatory or degenerative joint diseases into synovial fluid and serum.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 46 kDa.
Use a concentration of 1 µg/ml.
RelevanceAggrecan is a member of a family of large, aggregating proteoglycans (also including versican, brevican and neurocan) which is found in articular cartilage. Aggrecan is composed of three major domains: G1, G2, and G3. Between the G1 and G2 domains there is an interglobulin region (IGD). The IGD region is the major site of cleavage by specific proteases like metalloproteinases (MMPs) and aggrecanase. Aggrecan cleavage has been associated with a number of degenerative diseases including rheumatoid arthritis and osteoarthritis. There is evidence that this family of proteoglycans modulates cell adhesion, migration, and axonal outgrowth in the CNS.