Overview

  • Product name
  • Description
    Rabbit polyclonal to Aggrecan
  • Specificity
    ab53731 detects endogenous levels of fragment of activated Aggrecan resulting from cleavage adjacent to Asp369. It reacts with a cleaved (N-part) form of Aggrecan when it is cleaved at D369 of human aggrecan and at D369 of rat aggrecan. It will not react with full protein. The immunogen peptide is located at the C-term of the cleaved form.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide corresponding to Human Aggrecan.

  • Positive control
    • WB: Jurkat cell extracts.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab53731 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab53731 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 97 kDa (predicted molecular weight: 250 kDa).

Detects a band of approximately 97 kDa for the fragment (predicted molecular weight for the full length protein: 250 kDa).

ELISA 1/20000.

Target

  • Relevance
    Aggrecan is a member of a family of large, aggregating proteoglycans (also including versican, brevican and neurocan) which is found in articular cartilage. Aggrecan is composed of three major domains: G1, G2, and G3. Between the G1 and G2 domains there is an interglobulin region (IGD). The IGD region is the major site of cleavage by specific proteases like metalloproteinases (MMPs) and aggrecanase. Aggrecan cleavage has been associated with a number of degenerative diseases including rheumatoid arthritis and osteoarthritis. There is evidence that this family of proteoglycans modulates cell adhesion, migration, and axonal outgrowth in the CNS.
  • Cellular localization
    Secreted; extracellular matrix.
  • Database links
  • Alternative names
    • ACAN antibody
    • AGC 1 antibody
    • AGC1 antibody
    • AGCAN antibody
    • Aggrecan 1 (chondroitin sulfate proteoglycan 1, large aggregating proteoglycan, antigen identified by monoclonal antibody A0122) antibody
    • Aggrecan 1 antibody
    • Aggrecan core protein antibody
    • Aggrecan proteoglycan antibody
    • Aggrecan structural proteoglycan of cartilage antibody
    • Aggrecan1 antibody
    • ATEGQV antibody
    • Cartilage specific proteoglycan core protein antibody
    • Chondroitin sulfate proteoglycan 1 antibody
    • Chondroitin sulfate proteoglycan 1 large aggregating proteoglycan antigen identified by monoclonal antibody A0122 antibody
    • Chondroitin sulfate proteoglycan core protein 1 antibody
    • CSPG 1 antibody
    • CSPG1 antibody
    • CSPGCP antibody
    • JSCATE antibody
    • Large aggregating proteoglycan antibody
    • mcspg antibody
    • mgsk16 antibody
    • MSK 16 antibody
    • MSK16 antibody
    • SEDK antibody
    see all

Images

  • All lanes : Anti-Aggrecan antibody (ab53731) at 1/500 dilution

    Lane 1 : Jurkat cell extracts treated with Etoposide (25µM, 60mins)
    Lane 2 : Jurkat cell extracts treated with Etoposide (25µM, 60mins) with immunising peptide


    Predicted band size : 250 kDa
    Observed band size : 97 kDa (why is the actual band size different from the predicted?)
    Note: the observed band size may be smaller than predicted because this antibody detects endogenous levels of a fragment of activated Aggrecan.

References

This product has been referenced in:
  • Yin W  et al. Comparative evaluation of the effects of platelet-rich plasma formulations on extracellular matrix formation and the NF-?B signaling pathway in human articular chondrocytes. Mol Med Rep 15:2940-2948 (2017). WB ; Human . Read more (PubMed: 28339078) »

See 1 Publication for this product

Customer reviews and Q&As

Thank you for your patience in this matter. The originator of ab53731 (Aggrecan antibody - Cleaved Asp369) has informed us that this antibody reacts with a cleaved form of Aggrecan (N-terminal part) when it is cleaved at D369 of human/rat aggrecan. ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up