Overview

  • Product nameAnti-Aggrecan antibody
    See all Aggrecan primary antibodies
  • Description
    Rabbit polyclonal to Aggrecan
  • Specificityab53731 detects endogenous levels of fragment of activated Aggrecan resulting from cleavage adjacent to Asp369. It reacts with a cleaved (N-part) form of Aggrecan when it is cleaved at D369 of human aggrecan and at D369 of rat aggrecan. It will not react with full protein. The immunogen peptide is located at the C-term of the cleaved form.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide corresponding to Human Aggrecan.

  • Positive control
    • WB: Jurkat cell extracts.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab53731 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 97 kDa (predicted molecular weight: 250 kDa).

Detects a band of approximately 97 kDa for the fragment (predicted molecular weight for the full length protein: 250 kDa).

ELISA 1/20000.

Target

  • RelevanceAggrecan is a member of a family of large, aggregating proteoglycans (also including versican, brevican and neurocan) which is found in articular cartilage. Aggrecan is composed of three major domains: G1, G2, and G3. Between the G1 and G2 domains there is an interglobulin region (IGD). The IGD region is the major site of cleavage by specific proteases like metalloproteinases (MMPs) and aggrecanase. Aggrecan cleavage has been associated with a number of degenerative diseases including rheumatoid arthritis and osteoarthritis. There is evidence that this family of proteoglycans modulates cell adhesion, migration, and axonal outgrowth in the CNS.
  • Cellular localizationSecreted; extracellular matrix.
  • Database links
  • Alternative names
    • ACAN antibody
    • AGC 1 antibody
    • AGC1 antibody
    • AGCAN antibody
    • Aggrecan 1 (chondroitin sulfate proteoglycan 1, large aggregating proteoglycan, antigen identified by monoclonal antibody A0122) antibody
    • Aggrecan 1 antibody
    • Aggrecan core protein antibody
    • Aggrecan proteoglycan antibody
    • Aggrecan structural proteoglycan of cartilage antibody
    • Aggrecan1 antibody
    • ATEGQV antibody
    • Cartilage specific proteoglycan core protein antibody
    • Chondroitin sulfate proteoglycan 1 antibody
    • Chondroitin sulfate proteoglycan 1 large aggregating proteoglycan antigen identified by monoclonal antibody A0122 antibody
    • Chondroitin sulfate proteoglycan core protein 1 antibody
    • CSPG 1 antibody
    • CSPG1 antibody
    • CSPGCP antibody
    • JSCATE antibody
    • Large aggregating proteoglycan antibody
    • mcspg antibody
    • mgsk16 antibody
    • MSK 16 antibody
    • MSK16 antibody
    • SEDK antibody
    see all

Anti-Aggrecan antibody images

  • All lanes : Anti-Aggrecan antibody (ab53731) at 1/500 dilution

    Lane 1 : Jurkat cell extracts treated with Etoposide (25µM, 60mins)
    Lane 2 : Jurkat cell extracts treated with Etoposide (25µM, 60mins) with immunising peptide


    Predicted band size : 250 kDa
    Observed band size : 97 kDa (why is the actual band size different from the predicted?)
    Note: the observed band size may be smaller than predicted because this antibody detects endogenous levels of a fragment of activated Aggrecan.

References for Anti-Aggrecan antibody (ab53731)

ab53731 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your patience in this matter. The originator of ab53731 (Aggrecan antibody - Cleaved Asp369) has informed us that this antibody reacts with a cleaved form of Aggrecan (N-terminal part) when it is cleaved at D369 of human/rat aggrecan. ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"