Anti-Aggrecan ARGxx antibody [BC-3] (ab3773)

Overview

  • Product nameAnti-Aggrecan ARGxx antibody [BC-3]
  • Description
    Mouse monoclonal [BC-3] to Aggrecan ARGxx
  • SpecificityRecognises the aggrecanase (ADAMTS-1, -4 & -5)-generated N-terminal neoepitope ARG after cleavage between amino acids EGE and ARG within the interglobular domain of aggrecanase-catabolised aggrecan (Human aggrecan sequence enumeration). This antibody will not recognise the sequence ...ARG.. if it is in the non-cleaved intact aggrecan protein core; i.e. it will only recognise the aggrecanase generated neoepitope ARG... Samples need to be deglycosylated for epitope recognition (see notes).
  • Tested applicationsSuitable for: ELISA, IHC-P, IHC-Fr, WB, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Rat, Sheep, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Human, Pig
    Does not react with: Mouse
  • Immunogen

    ARGSV

    ... synthetic peptide conjugate.

  • General notesSamples must be deglycosylated using 0.01 Units Chondroitinase ABC (Sigma), 0.01 Units Keratanase (Seikagaku) and 0.0001 Units Keratanase II (Seikagaku) per 10µg S-GAG of non-deglycosylated aggrecan for optimal epitope recognition. See Little et al. and Caterson et al. Diluted Technomouse culture supernatant.


    Aggrecan degradation products containing this neoepitope are rapidly released from the tissue in model explant culture systems and are also present in the synovial fluids of patients with degenerative joint disease.

Properties

Applications

Our Abpromise guarantee covers the use of ab3773 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

This antibody should work in IHC on formalin- or paraformaldehyde-fixed paraffin embedded sections.

IHC-Fr Use at an assay dependent concentration.

This antibody should work in IHC on alcohol-fixed frozen sections or un-fixed snap-frozen sections.

WB 1/100. Detects a band of approximately 50-250 kDa.

Detects a variety of epitopes between 50 and 250 kDa.

Sandwich ELISA Use at an assay dependent concentration. PubMed: 19932675

Target

References for Anti-Aggrecan ARGxx antibody [BC-3] (ab3773)

This product has been referenced in:
  • Lu W  et al. CXCL12/CXCR4 Axis Regulates Aggrecanase Activation and Cartilage Degradation in a Post-Traumatic Osteoarthritis Rat Model. Int J Mol Sci 17:N/A (2016). Rat . Read more (PubMed: 27690009) »
  • Kumar S  et al. Loss of ADAMTS4 reduces high fat diet-induced atherosclerosis and enhances plaque stability in ApoE(-/-) mice. Sci Rep 6:31130 (2016). IHC-Fr ; Mouse . Read more (PubMed: 27491335) »

See all 17 Publications for this product

Product Wall



Nicht aufgereinigte Antikörper wie Zellkulturüberstand, Vollserum oder Aszites haben keine determinierte Konzentration und können stark in Ihrer Konzentration schwanken.

Wenn die spezifische Antikörperkonzentrati...

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TSC - was cultured in serum?
A: Serum was used during culture.

is the Ab available in purified form?
A: We do not routinely purify the antibody and so cannot give an answer to the second question. However, it could be purified.
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Thank you for your inquiry. Please find a list of possible positive controls below: ab2426: skin, lymph nodes Click here (or use the following: http://www.abcam.com/index.html?datasheet=2426). ab3773: cartilage samples Click here (or use the followin...

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I have been informed of the following information which I hope will help you: Ab3773: this should be reactive with goat since this antibody recognises a sequence ARG... that is conserved in all species Guinea Pig/rat to human but not mouse for some ...

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Thank you for your enquiry. The following reference gives details of the protocol used with this antibody in immunohistochemistry: Roberts S et al. Matrix metalloproteinases and aggrecanase: their role in disorders of the human intervertebral disc....

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application ELISA
Sample Human Recombinant protein (Part of aggrecan peptide 41AA)
Specification Part of aggrecan peptide 41AA
Type Indirect
Username

Abcam user community

Verified customer

Submitted Nov 29 2006

Thank you for your patience. I can see that my colleague has also written to you and referred you to the paper in which the antibody was tested, i.e. Little CB et al., Matrix Biol 21:271-88 (2002). In summary the antibody detects a variety of epitope...

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The antibody ab3773 was tested in western blot analysis on proteoglycan metabolites released into the culture media and extracted from the cartilage matrix in cultures of bovine nasal cartilage maintained for 21 days in the paper Little CB et al. Matr...

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Thank you for getting back in touch with me. I have been in touch with the originator of this antibody. They have provided me with some further details. They mention that both ab3773 & ab3776 are not usually used for IHC studies of articular cartila...

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Thank you for getting back to me and highlighting these details on our datasheet. I can now appreciate the deglycosylation that you have performed in line with the following publication. Little CB et al. Matrix metalloproteinases are involved in C-t...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"