Overview

  • Product nameAnti-AGL antibody
    See all AGL primary antibodies
  • Description
    Mouse polyclonal to AGL
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length Human AGL protein (NP_000019.2, 1 a.a. ~ 1532 a.a).

  • Positive control
    • Human liver lysate. AGL transfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab72770 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 175 kDa.

Target

  • FunctionMultifunctional enzyme acting as 1,4-alpha-D-glucan:1,4-alpha-D-glucan 4-alpha-D-glycosyltransferase and amylo-1,6-glucosidase in glycogen degradation.
  • Tissue specificityLiver, kidney and lymphoblastoid cells express predominantly isoform 1; whereas muscle and heart express not only isoform 1, but also muscle-specific isoform mRNAs (isoforms 2, 3 and 4). Isoforms 5 and 6 are present in both liver and muscle.
  • Involvement in diseaseGlycogen storage disease 3
  • Sequence similaritiesBelongs to the glycogen debranching enzyme family.
  • Post-translational
    modifications
    The N-terminus is blocked.
    Ubiquitinated.
  • Cellular localizationCytoplasm. Under glycogenolytic conditions localizes to the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1110061O17Rik antibody
    • 4-1 antibody
    • 4-glucantransferase antibody
    • 6-glucosidase antibody
    • 9430004C13Rik antibody
    • 9630046L06Rik antibody
    • AGL antibody
    • AI850929 antibody
    • Amylo 1 6 glucosidase 4 alpha glucanotransferase antibody
    • Amylo-1 antibody
    • Amylo-alpha-1 antibody
    • C77197 antibody
    • Dextrin 6-alpha-D-glucosidase antibody
    • GDE antibody
    • GDE_HUMAN antibody
    • Glycogen debrancher antibody
    • Glycogen debranching enzyme antibody
    • Glycogen storage disease type III antibody
    • Oligo-1 antibody
    see all

Anti-AGL antibody images

  • Anti-AGL antibody (ab72770) at 1 µg/ml + Human liver lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 175 kDa
    Observed band size : 150 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-AGL antibody (ab72770) at 1 µg/ml

    Lane 1 : AGL transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 175 kDa
    Observed band size : 175 kDa

References for Anti-AGL antibody (ab72770)

ab72770 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab72770.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"