Overview

  • Product nameAnti-Ago1 antibody
    See all Ago1 primary antibodies
  • Description
    Rabbit polyclonal to Ago1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Schizosaccharomyces pombe
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 350 - 450 of Schizosaccharomyces pombe Ago1.

  • Positive control
    • This antibody gave a positive signal in S. Pombe whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab18190 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 95 kDa).

Target

  • FunctionRequired for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Also required for transcriptional gene silencing (TGS) of promoter regions which are complementary to bound short antigene RNAs (agRNAs).
  • Sequence similaritiesBelongs to the argonaute family. Ago subfamily.
    Contains 1 PAZ domain.
    Contains 1 Piwi domain.
  • Cellular localizationCytoplasm > P-body.
  • Information by UniProt
  • Database links
    • Alternative names
      • AGO1 antibody
      • AGO1_HUMAN antibody
      • Argonaute 1 antibody
      • Argonaute1 antibody
      • eIF 2C 1 antibody
      • eIF-2C 1 antibody
      • eIF2C 1 antibody
      • EIF2C antibody
      • Eif2c1 antibody
      • Eukaryotic translation initiation factor 2C 1 antibody
      • GERP95 antibody
      • Golgi Endoplasmic Reticulum protein 95 kDa antibody
      • hAgo1 antibody
      • Protein argonaute 1 antibody
      • Protein argonaute-1 antibody
      • Putative RNA binding protein Q99 antibody
      • Putative RNA-binding protein Q99 antibody
      • Q99 antibody
      see all

    Anti-Ago1 antibody images

    • Anti-Ago1 antibody (ab18190) at 1 µg/ml + S.pombe Whole Cell Lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Predicted band size : 95 kDa
      Observed band size : 85 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 16 kDa,250 kDa,90 kDa. We are unsure as to the identity of these extra bands.
      Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
    • All lanes : Anti-Ago1 antibody (ab18190) at 1 µg/ml

      Lane 1 : S.pombe wild-type whole cell extract
      Lane 2 : S.pombe ago1Δ whole cell extract
      Lane 3 : S.pombe endogenously expressed tagged Ago1 whole cell extract
      Lane 4 : S.pombe over-expressed tagged Ago1 whole cell extract
      Lane 5 : S.pombe repressed tagged Ago1 whole cell extract

      Lysates/proteins at 20 µg per lane.


      Predicted band size : 95 kDa
      Observed band size : 95 kDa
      Additional bands at : 60 kDa (possible cross reactivity).

      This image is courtesy of Shane Buker, Department of Cell Biology, Harvard

      ab18190 faintly recognises Ago1 in the wildtype and expressed tagged Ago1 strains at 95kDa but not in the Ago1Δ or repressed tagged Ago1 as expected. There is a non-specific band at 60kDa, which attributed to cross-reactivity.

    References for Anti-Ago1 antibody (ab18190)

    This product has been referenced in:
    • Shah S  et al. lncRNA recruits RNAi and the exosome to dynamically regulate pho1 expression in response to phosphate levels in fission yeast. Genes Dev 28:231-44 (2014). ChIP ; Schizosaccharomyces pombe . Read more (PubMed: 24493644) »
    • Partridge JF  et al. Functional separation of the requirements for establishment and maintenance of centromeric heterochromatin. Mol Cell 26:593-602 (2007). Read more (PubMed: 17531816) »

    See all 3 Publications for this product

    Product Wall

    Thank you for your interest in our products.

    In order to be able to help you further, would you be so kind to confirm what issue you have experienced with ab18190 and why you would need a specific protocol for preparing yeast lysates?
    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"