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Synthetic peptide conjugated to KLH derived from within residues 350 - 450 of Schizosaccharomyces pombe Ago1.
Our Abpromise guarantee covers the use of ab18190 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 95 kDa).|
This image is courtesy of Shane Buker, Department of Cell Biology, Harvard
ab18190 faintly recognises Ago1 in the wildtype and expressed tagged Ago1 strains at 95kDa but not in the Ago1Δ or repressed tagged Ago1 as expected. There is a non-specific band at 60kDa, which attributed to cross-reactivity.