Overview

  • Product name
  • Description
    Rabbit polyclonal to Agpat2
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cat, Pig
  • Immunogen

    A region within synthetic peptide: IPTSGLTAAD VPALVDTCHR AMRTTFLHIS KTPQENGATA GSGVQPAQ, corresponding to C terminal amino acids 199-246 of Human Agpat2

  • Positive control
    • Jurkat cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab62599 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 5 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).Can be blocked with ab185536. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

Target

  • Function
    Converts lysophosphatidic acid (LPA) into phosphatidic acid by incorporating an acyl moiety at the sn-2 position of the glycerol backbone.
  • Tissue specificity
    Expressed predominantly in heart and liver.
  • Pathway
    Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 2/3.
  • Involvement in disease
    Defects in AGPAT2 are the cause of congenital generalized lipodystrophy type 1 (CGL1) [MIM:608594]; also known as Berardinelli-Seip congenital lipodystrophy type 1 (BSCL1) or Berardinelli-Seip syndrome. CGL1 is an autosomal recessive disorder characterized by marked paucity of adipose tissue, extreme insulin resistance, hypertriglyceridemia, hepatic steatosis and early onset of diabetes.
  • Sequence similarities
    Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.
  • Domain
    The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1 acyl sn glycerol 3 phosphate acyltransferase beta antibody
    • 1 acylglycerol 3 phosphate O acyltransferase 2 antibody
    • 1 AGP acyltransferase 2 antibody
    • 1 AGPAT2 antibody
    • 1-acyl-sn-glycerol-3-phosphate acyltransferase beta antibody
    • 1-acylglycerol-3-phosphate O-acyltransferase 2 antibody
    • 1-AGP acyltransferase 2 antibody
    • 1-AGPAT 2 antibody
    • Agpat2 antibody
    • Berardinelli Seip congenital lipodystrophy antibody
    • BSCL antibody
    • BSCL1 antibody
    • EC 2.3.1.51 antibody
    • LPAAB antibody
    • LPAAT beta antibody
    • LPAAT-beta antibody
    • Lysophosphatidic acid acyltransferase beta antibody
    • PLCB_HUMAN antibody
    see all

Anti-Agpat2 antibody images

  • Lane 1 : Molecular weight marker
    Lane 2 : Anti-Agpat2 antibody (ab62599) at 5 µg/ml

    Lane 1 : As above
    Lane 2 : Jurkat cell lysate at 10 µg

    Secondary
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution


    Predicted band size : 31 kDa
    Observed band size : 31 kDa

References for Anti-Agpat2 antibody (ab62599)

This product has been referenced in:
  • Zhang C  et al. Glycerolipid signals alter mTOR complex 2 (mTORC2) to diminish insulin signaling. Proc Natl Acad Sci U S A 109:1667-72 (2012). Read more (PubMed: 22307628) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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