Overview

  • Product nameAnti-AGR3 antibody [AGR3.1]
    See all AGR3 primary antibodies
  • Description
    Mouse monoclonal [AGR3.1] to AGR3
  • Tested applicationsSuitable for: WB, IHC-P, IHC-Fr, ICCmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Purified human AGR3 protein

  • EpitopeHETTDKNLS within the AGR3 (AG3) protein
  • Positive control
    • T47D breast cancer cell line and H1229 lung carcinoma cell line (WB and ICC). For negative control use H1299 lung carcinoma cell line. Breast cancer tissue (IHC)

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: 0.2% high-grade protease free BSA, PBS
  • Concentration information loading...
  • PurityProtein A purified
  • Purification notesPurified from ascites by protein A affinity chromatography.
  • ClonalityMonoclonal
  • Clone numberAGR3.1
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab82400 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 19 kDa.
IHC-P Use a concentration of 1 µg/ml.
IHC-Fr Use a concentration of 1 µg/ml.
ICC Use a concentration of 1 µg/ml.

Target

  • Sequence similaritiesBelongs to the AGR family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • AG-3 antibody
    • AG3 antibody
    • Agr3 antibody
    • AGR3_HUMAN antibody
    • Anterior gradient 3, xenopus, homolog of antibody
    • Anterior gradient homolog 3 antibody
    • Anterior gradient homolog 3 (Xenopus laevis) antibody
    • anterior gradient protein 3 antibody
    • Anterior gradient protein 3 homolog antibody
    • BCMP11 antibody
    • Breast cancer membrane protein 11 antibody
    • Gm888 antibody
    • hAG 3 antibody
    • hAG-3 antibody
    • HAG3 antibody
    • OTTHUMP00000158512 antibody
    • PDIA18 antibody
    • Protein disulfide isomerase family A member 18 antibody
    see all

Anti-AGR3 antibody [AGR3.1] images

  • Immunohistochemistry staining of AGR3 with ab82400 at 1ug/ml in paraffin-embedded sections of breast carcinoma.
  • All lanes : Anti-AGR3 antibody [AGR3.1] (ab82400) at 1 µg/ml

    Lane 1 : H1229 lung carcinoma cell line
    Lane 2 : T47D breast cancer cell line


    Predicted band size : 19 kDa
  • ICC/IF image of ab82400 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab82400, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-AGR3 antibody [AGR3.1] (ab82400)

This product has been referenced in:
  • Garczyk S  et al. AGR3 in breast cancer: prognostic impact and suitable serum-based biomarker for early cancer detection. PLoS One 10:e0122106 (2015). WB, IHC-P ; Human . Read more (PubMed: 25875093) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"