Anti-Agrin antibody (ab85174)

Overview

  • Product nameAnti-Agrin antibody
    See all Agrin primary antibodies
  • Description
    Rabbit polyclonal to Agrin
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide from the N terminal residues of Human Agrin protein (NM_198576).

  • Positive control
    • Human skeletal muscle.

Properties

Applications

Our Abpromise guarantee covers the use of ab85174 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500.

Target

  • RelevanceAgrin is an essential extracellular matrix component which promotes clustering of nicotinic acetylcholine receptors (nAChRs) and other proteins during development at the neuromuscular junction. Agrin, MuSK and Rapsyn are all essential components for AChR aggregation, through an unknown mechanism. The C-terminal region of agrin is released into the medium, interacts with receptors on the muscle surface and induces AChR aggregation. The central region contains two O-linked glycosylation sites and a domain homologous to domain III of laminin. The N-terminal region anchors agrin to the extracellular matrix via other basal membrane components. This region also contains a protease inhibitor domain and glycosaminoglycan attachment sites; increasing the predicted MW from 200kDa to ~600kDa.
  • Cellular localizationSecreted; extracellular space; extracellular matrix. Note: Synaptic basal lamina at the neuromuscular junction.
  • Database links
  • Alternative names
    • AGRIN antibody
    • Agrin proteoglycan antibody
    • AGRN antibody
    • FLJ45064 antibody
    • OTTHUMP00000044043 antibody
    see all

Anti-Agrin antibody images

  • ab85174 staining Agrin in foetal colon tissue by immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) at a dilution of 1/100.

  • ab85174, at 1/100 dilution, staining Agrin in formalin-fixed, paraffin-embedded human skeletal muscle by Immunohistochemistry.

References for Anti-Agrin antibody (ab85174)

ab85174 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Rat embryonic Dorsal Horn Cells in culture)
Permeabilization No
Specification Rat embryonic Dorsal Horn Cells in culture
Fixative 4%PF,0.2% Picric Acid
Username

Ms. Babben Tinner

Verified customer

Submitted Oct 13 2015

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (4-12%)
Sample Human Purified protein (proteoglycan preparation from human milk)
Specification proteoglycan preparation from human milk
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. serena leone

Verified customer

Submitted Dec 04 2013

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5%
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium Citrate Buffer & Tris pH 9.0
Sample Mouse Tissue sections (mouse kidney)
Specification mouse kidney
Permeabilization Yes - 0.2 % Triton X in PBS
Fixative Formaldehyde
Username

Dr. Steffen Rickelt

Verified customer

Submitted Nov 12 2013

The immunogen is a 14 amino acid peptide. It is within this range of amino acids: magrshpgpl rpllpllvva acvlpgaggt cperalerre eeanvvltgt veeilnvdpv.

Thank you for you enquiry regarding ab85174.

As our Abpromise indicates, in the event that a product is not functioning in the applications/species cited on the product data sheet (and the problem has been reported within 6 months of purchase...

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Thank you for contacting Abcam.   I have check with the lab and they have confimred that the immunogen sequence is within the first 1-250aa of the protein. They are unable to provide the actual sequence of the immunogen but I hope that this information...

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