Overview

  • Product nameAnti-Agrin antibody [AGR 131]
    See all Agrin primary antibodies
  • Description
    Mouse monoclonal [AGR 131] to Agrin
  • SpecificityAb12362 detects all isoforms of agrin. This antibody recognizes all N1 isoforms but, consistent with the epitope mapping investigations, fails to see the N4 protein. Furthermore, the antibody will detect the X, Y and Z splice variants.
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Recombinant rat agrin, C-terminal construct.

  • EpitopeEpitope is located near the 1st EGF-like repeat.

Properties

Applications

Our Abpromise guarantee covers the use of ab12362 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 208.6 kDa.

Target

  • RelevanceAgrin is an essential extracellular matrix component which promotes clustering of nicotinic acetylcholine receptors (nAChRs) and other proteins during development at the neuromuscular junction. Agrin, MuSK and Rapsyn are all essential components for AChR aggregation, through an unknown mechanism. The C-terminal region of agrin is released into the medium, interacts with receptors on the muscle surface and induces AChR aggregation. The central region contains two O-linked glycosylation sites and a domain homologous to domain III of laminin. The N-terminal region anchors agrin to the extracellular matrix via other basal membrane components. This region also contains a protease inhibitor domain and glycosaminoglycan attachment sites; increasing the predicted MW from 200kDa to ~600kDa.
  • Cellular localizationSecreted; extracellular space; extracellular matrix. Note: Synaptic basal lamina at the neuromuscular junction.
  • Database links
  • Alternative names
    • AGRIN antibody
    • Agrin proteoglycan antibody
    • AGRN antibody
    • FLJ45064 antibody
    • OTTHUMP00000044043 antibody
    see all

References for Anti-Agrin antibody [AGR 131] (ab12362)

This product has been referenced in:
  • Smuder AJ  et al. Cervical spinal cord injury exacerbates ventilator-induced diaphragm dysfunction. J Appl Physiol (1985) 120:166-77 (2016). Read more (PubMed: 26472866) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"