Overview

  • Product name
    Anti-Agrin antibody [Agr 247]
    See all Agrin primary antibodies
  • Description
    Mouse monoclonal [Agr 247] to Agrin
  • Specificity
    Ab12364 regognises agrin.
  • Tested applications
    Suitable for: ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Recombinant rat agrin, C-terminal construct.

  • Epitope
    Ab12364 binds to an epitope close to splicing site Z and causes a marked reduction in the number of nicotinic acetylcholine receptor(nAChR) clusters in samples from mouse and rat origins.
  • Positive control
    • Mouse Brain Tissue Extract.
  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab12364 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use a concentration of 2 µg/ml.

Target

  • Relevance
    Agrin is an essential extracellular matrix component which promotes clustering of nicotinic acetylcholine receptors (nAChRs) and other proteins during development at the neuromuscular junction. Agrin, MuSK and Rapsyn are all essential components for AChR aggregation, through an unknown mechanism. The C-terminal region of agrin is released into the medium, interacts with receptors on the muscle surface and induces AChR aggregation. The central region contains two O-linked glycosylation sites and a domain homologous to domain III of laminin. The N-terminal region anchors agrin to the extracellular matrix via other basal membrane components. This region also contains a protease inhibitor domain and glycosaminoglycan attachment sites; increasing the predicted MW from 200kDa to ~600kDa.
  • Cellular localization
    Secreted; extracellular space; extracellular matrix. Note: Synaptic basal lamina at the neuromuscular junction.
  • Database links
  • Alternative names
    • AGRIN antibody
    • Agrin proteoglycan antibody
    • AGRN antibody
    • FLJ45064 antibody
    • OTTHUMP00000044043 antibody
    see all

References

This product has been referenced in:
  • Tourovskaia A  et al. Local induction of acetylcholine receptor clustering in myotube cultures using microfluidic application of agrin. Biophys J 90:2192-8 (2006). Read more (PubMed: 16387765) »

See 1 Publication for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris pH 9.0
Sample
Mouse Tissue sections (mouse kidney)
Specification
mouse kidney
Permeabilization
Yes - 0.2% Triton X-100 and PBS
Fixative
Formaldehyde
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Verified customer

Submitted Mar 14 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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