Overview

  • Product nameAnti-AGRP antibody
    See all AGRP primary antibodies
  • Description
    Rabbit polyclonal to AGRP
  • Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide from internal sequence residues of Human AGRP (NM_001138.1).

  • Positive control
    • Human fetal brain and fetal testis lysates. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HeLa.

Properties

Applications

Our Abpromise guarantee covers the use of ab113481 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. PubMed: 24944903
ICC/IF Use a concentration of 10 µg/ml.
WB 1/500 - 1/1000. Predicted molecular weight: 14 kDa.

Target

  • FunctionPlays a role in weight homeostasis. Plays a role in the central control of feeding. Reduces food intake. Inhibits cAMP production mediated by stimulation of melanocortin receptors within the hypothalamus and adrenal gland. Acts primarily on MC3R and MC4R. Has very low activity with MC5R.
  • Tissue specificityExpressed primarily in the adrenal gland, subthalamic nucleus, and hypothalamus, with a lower level of expression occurring in testis, lung, and kidney.
  • Involvement in diseaseGenetic variations in AGRP may be a cause of obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.
  • Sequence similaritiesContains 1 agouti domain.
  • DomainThe presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.
  • Cellular localizationSecreted. Golgi apparatus lumen.
  • Information by UniProt
  • Database links
  • Alternative names
    • Agouti related neuropeptide antibody
    • Agouti Related Protein Homolog antibody
    • Agouti-related protein antibody
    • Agrp antibody
    • AGRP_HUMAN antibody
    • AGRT antibody
    • ART antibody
    • ASIP2 antibody
    see all

Anti-AGRP antibody images

  • ICC/IF image of ab113481 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab113481 at 10µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • All lanes : Anti-AGRP antibody (ab113481) at 1/500 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Human fetal testis lysate


    Predicted band size : 14 kDa

References for Anti-AGRP antibody (ab113481)

This product has been referenced in:
  • Imbernon M  et al. Hypothalamic KLF4 mediates leptin's effects on food intake via AgRP. Mol Metab 3:441-51 (2014). IHC-P ; Rat . Read more (PubMed: 24944903) »
  • Lopez R  et al. Hyperglycemia enhances the proliferation of non-tumorigenic and malignant mammary epithelial cells through increased leptin/IGF1R signaling and activation of AKT/mTOR. PLoS One 8:e79708 (2013). WB ; Human . Read more (PubMed: 24260287) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"