Overview

  • Product name
  • Description
    Mouse polyclonal to AGTRAP
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length Human AGTRAP protein (NP_001035284.1, amino acids 1-152)

  • Positive control
    • HeLa nuclear extract and AGTRAP transfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab89247 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 17 kDa.
IHC-P Use at an assay dependent concentration.

Target

  • Information by UniProt
  • Database links
  • Alternative names
    • Agtrap antibody
    • Angiotensin II receptor associated protein antibody
    • Angiotensin II type I receptor associated protein antibody
    • AT1 receptor-associated protein antibody
    • ATI receptor associated protein antibody
    • ATRAP antibody
    • ATRAP_HUMAN antibody
    • MGC29646 antibody
    • OTTHUMP00000002269 antibody
    • OTTHUMP00000002270 antibody
    • OTTHUMP00000002271 antibody
    • Type 1 angiotensin II receptor associated protein antibody
    • Type-1 angiotensin II receptor-associated protein antibody
    see all

Anti-AGTRAP antibody images

  • Anti-AGTRAP antibody (ab89247) at 1 µg/ml + HeLa nuclear extract at 50 µg

    Predicted band size : 17 kDa
    Observed band size : 19 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-AGTRAP antibody (ab89247) at 1 µg/ml

    Lane 1 : AGTRAP transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate.

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 17 kDa
    Observed band size : 17 kDa

References for Anti-AGTRAP antibody (ab89247)

ab89247 has not yet been referenced specifically in any publications.

Product Wall

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (kidney)
Specification
kidney
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
No
Blocking step
Blocking reagens Fa.Zytomed as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
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Verified customer

Submitted Mar 01 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rabbit Tissue sections (eye)
Specification
eye
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
No
Blocking step
Fa.Zytomed as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Mar 01 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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