Overview

  • Product nameAnti-AGXT antibody [1F9BC7]
    See all AGXT primary antibodies
  • Description
    Mouse monoclonal [1F9BC7] to AGXT
  • Tested applicationsSuitable for: ICC/IF, IP, In-Cell ELISAmore details
  • Species reactivity
    Reacts with: Rat, Cow, Human
  • Immunogen

    Human liver mitochondria

  • Positive control
    • HepG2 cells; Human and Rat liver, Bovine heart and HepG2 cell lysates.
  • General notes

    Product was previously marketed under the MitoSciences sub-brand.

Properties

Applications

Our Abpromise guarantee covers the use of ab110313 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IP Use at an assay dependent dilution.
In-Cell ELISA Use a concentration of 5 µg/ml.

Target

  • Tissue specificityLiver.
  • Involvement in diseaseDefects in AGXT are the cause of hyperoxaluria primary type 1 (HP1) [MIM:259900]; also known as primary hyperoxaluria type I (PH1) and oxalosis I. HP1 is a rare autosomal recessive inborn error of glyoxylate metabolism characterized by increased excretion of oxalate and glycolate, and the progressive accumulation of insoluble calcium oxalate in the kidney and urinary tract.
  • Sequence similaritiesBelongs to the class-V pyridoxal-phosphate-dependent aminotransferase family.
  • Cellular localizationPeroxisome. Mitochondrion matrix. Except in some HP1 patients where AGT is found in the mitochondrial matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • AGT antibody
    • AGT1 antibody
    • Agxt antibody
    • AGXT1 antibody
    • Alanine glyoxylate aminotransferase antibody
    • Alanine glyoxylate aminotransferase3 antibody
    • Alanine--glyoxylate aminotransferase antibody
    • EC 2.6.1.44 antibody
    • EC 2.6.1.51 antibody
    • Hepatic peroxisomal alanine glyoxylate aminotransferase antibody
    • Hepatic peroxisomal alanine:glyoxylate aminotransferase antibody
    • L alanine glyoxylate aminotransferase 1 antibody
    • MS773 antibody
    • PH1 antibody
    • Serine pyruvate aminotransferase antibody
    • Serine--pyruvate aminotransferase antibody
    • Serine--pyruvate aminotransferase, mitochondrial antibody
    • Serine:pyruvate aminotransferase antibody
    • SPAT antibody
    • SPT antibody
    • SPYA_HUMAN antibody
    • TLH6 antibody
    see all

Anti-AGXT antibody [1F9BC7] images

  • Immunocytochemistry analysis using ab110313 at 5 ug/ml staining AGXT in HepG2 cells (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (green).
  • Using MitoSciences' standard immunoprecipitation protocol, ab110313 precipicates with the 43kDa AGXT protein in human liver samples. Very small amounts of AGXT are immunoprecipitated from bovine heart, rat liver or human HepG2 cultured cell lysate. Identity of this protein was confirmed by mass spectrometry. This gel was stained with silver.

References for Anti-AGXT antibody [1F9BC7] (ab110313)

ab110313 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"