Overview

  • Product nameAnti-AGXT antibody
    See all AGXT primary antibodies
  • Description
    Rabbit polyclonal to AGXT
  • Tested applicationsSuitable for: IHC-P, Flow Cyt, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human AGXT aa 96-125 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: P21549

  • Positive control
    • WB: HepG2 cell lysate. IHC-P: Human hepatocarcinoma tissue. Flow Cyt: HepG2 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab91074 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
Flow Cyt 1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
WB 1/1000. Predicted molecular weight: 43 kDa.

Target

  • Tissue specificityLiver.
  • Involvement in diseaseDefects in AGXT are the cause of hyperoxaluria primary type 1 (HP1) [MIM:259900]; also known as primary hyperoxaluria type I (PH1) and oxalosis I. HP1 is a rare autosomal recessive inborn error of glyoxylate metabolism characterized by increased excretion of oxalate and glycolate, and the progressive accumulation of insoluble calcium oxalate in the kidney and urinary tract.
  • Sequence similaritiesBelongs to the class-V pyridoxal-phosphate-dependent aminotransferase family.
  • Cellular localizationPeroxisome. Mitochondrion matrix. Except in some HP1 patients where AGT is found in the mitochondrial matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • AGT antibody
    • AGT1 antibody
    • Agxt antibody
    • AGXT1 antibody
    • Alanine glyoxylate aminotransferase antibody
    • Alanine glyoxylate aminotransferase3 antibody
    • Alanine--glyoxylate aminotransferase antibody
    • EC 2.6.1.44 antibody
    • EC 2.6.1.51 antibody
    • Hepatic peroxisomal alanine glyoxylate aminotransferase antibody
    • Hepatic peroxisomal alanine:glyoxylate aminotransferase antibody
    • L alanine glyoxylate aminotransferase 1 antibody
    • MS773 antibody
    • PH1 antibody
    • Serine pyruvate aminotransferase antibody
    • Serine--pyruvate aminotransferase antibody
    • Serine--pyruvate aminotransferase, mitochondrial antibody
    • Serine:pyruvate aminotransferase antibody
    • SPAT antibody
    • SPT antibody
    • SPYA_HUMAN antibody
    • TLH6 antibody
    see all

Anti-AGXT antibody images

  • Anti-AGXT antibody (ab91074) at 1/1000 dilution + HepG2 cell lysate at 35 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/5000 dilution

    Predicted band size : 43 kDa

    Incubation time was overnight at 4°C. Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocarcinoma tissue labelling AGXT with ab91074. A peroxidase-conjugated anti-rabbit IgG was used as the secondary antibody, followed by DAB staining.

  • Flow cytometry analysis of HepG2 cells labelling AGXT (green) with ab91074 compared to a negative control (blue). A FITC-conjugated goat anti-rabbit IgG was used as the secondary antibody.

References for Anti-AGXT antibody (ab91074)

ab91074 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab91074.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"