The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100.
1/10 - 1/50.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
1/1000. Predicted molecular weight: 43 kDa.
Involvement in disease
Defects in AGXT are the cause of hyperoxaluria primary type 1 (HP1) [MIM:259900]; also known as primary hyperoxaluria type I (PH1) and oxalosis I. HP1 is a rare autosomal recessive inborn error of glyoxylate metabolism characterized by increased excretion of oxalate and glycolate, and the progressive accumulation of insoluble calcium oxalate in the kidney and urinary tract.
Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family.
Peroxisome. Mitochondrion matrix. Except in some HP1 patients where AGT is found in the mitochondrial matrix.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocarcinoma tissue labelling AGXT with ab91074. A peroxidase-conjugated anti-rabbit IgG was used as the secondary antibody, followed by DAB staining.
Flow Cytometry - Anti-AGXT antibody (ab91074)
Flow cytometry analysis of HepG2 cells labelling AGXT (green) with ab91074 compared to a negative control (blue). A FITC-conjugated goat anti-rabbit IgG was used as the secondary antibody.