Anti-AHA1 antibody [25F2.D9] (ab91259)

Overview

  • Product name
    Anti-AHA1 antibody [25F2.D9]
    See all AHA1 primary antibodies
  • Description
    Rat monoclonal [25F2.D9] to AHA1
  • Tested applications
    Suitable for: WB, IP, ELISA, IHC-P, IHC-Fr, ICC, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Mouse AHA1

  • Positive control
    • Rat tissue lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    25F2.D9
  • Isotype
    IgG2a
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab91259 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
ELISA
IHC-P
IHC-Fr
ICC
ICC/IF
  • Application notes
    ELISA: Use at an assay dependent dilution.
    ICC: Use at an assay dependent dilution.
    ICC/IF: Use at an assay dependent dilution.
    IHC-P: Use at an assay dependent dilution.
    IHC-Fr: Use at an assay dependent dilution.
    IP: Use at an assay dependent dilution.
    WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 38 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Cochaperone that stimulates HSP90 ATPase activity (By similarity). May affect a step in the endoplasmic reticulum to Golgi trafficking.
    • Tissue specificity
      Expressed in numerous tissues, including brain, heart, skeletal muscle and kidney and, at lower levels, liver and placenta.
    • Sequence similarities
      Belongs to the AHA1 family.
    • Cellular localization
      Cytoplasm > cytosol. Endoplasmic reticulum. May transiently interact with the endoplasmic reticulum.
    • Information by UniProt
    • Database links
    • Alternative names
      • Activator of 90 kDa heat shock protein ATPase homolog 1 antibody
      • Activator of heat shock 90kDa protein ATPase homolog 1 antibody
      • AHA 1 antibody
      • AHA1 antibody
      • AHSA 1 antibody
      • Ahsa1 antibody
      • AHSA1_HUMAN antibody
      • C14orf3 antibody
      • HSPC322 antibody
      • p38 antibody
      see all

    Anti-AHA1 antibody [25F2.D9] images

    • Anti-AHA1 antibody [25F2.D9] (ab91259) at 1/1000 dilution + Human cell line lysate

      Predicted band size : 38 kDa
    • ab91259 staining AHA1 in HeLa cells by ICC/IF (Immunocytochemistry/immunofluoresence). Cells were fixed with 4% PFA for 20 minutes at room temperature. Samples were incubated with primary antibody (1/100) for 1 hour at room temperature. A Fluoroscein-conjugated goat anti-rat IgG secondary antibody was used.
    • ab91259 used in the Immunoprecipitation of AHA1 from Human Hela cells. The antibody was used at 20µl per 1 ml of cell lysate (50mM Tris (pH 7.2); 150mM NaCl; 1mM MgCl2; 1% TX-100; protease inhibitors); captured on 20µl of protein G beads .

    References for Anti-AHA1 antibody [25F2.D9] (ab91259)

    ab91259 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting us. We have 4 anti-AHA1 antibodies: ab91259, ab83036, ab56721 and ab64998. The first 3 antibodies were raised against the full length mouse or human protein, respectively. The sequence homology with these ...

    Read More

    Thank you for contacting us. Unfortunately, none of the antibodies you mentioned (ab91259, ab83036, ab13492) have been tested in zebrafish so far. However, we have another HSP90 antibody that was tested in zebrafish: ab13494 (http://w...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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