Overview

  • Product nameAnti-AHCYL1 antibody
    See all AHCYL1 primary antibodies
  • Description
    Mouse monoclonal to AHCYL1
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Chicken, Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 1-102 of Human AHCYL1

Properties

Applications

Our Abpromise guarantee covers the use of ab56761 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 56 kDa.
IHC-P Use a concentration of 3 µg/ml.
Flow Cyt Use 0.01-0.1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Target

  • Tissue specificityExpressed in dendritic cells.
  • PathwayAmino-acid biosynthesis; L-homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1.
  • Sequence similaritiesBelongs to the adenosylhomocysteinase family.
  • Developmental stageExpression increases markedly during activation of blood and skin DC (Langerhans cells), but is diminished in terminally differentiated tonsil DC.
  • DomainThe PEST region is essential for the interaction with ITPR1. The PDZ-binding region is required for maximal interaction with ITPR1 and is also responsible for the IP3-insensitive interaction with ITPR1.
  • Cellular localizationEndoplasmic reticulum.
  • Information by UniProt
  • Database links
  • Alternative names
    • AA409031 antibody
    • AdoHcyase 2 antibody
    • Ahcy-rs3 antibody
    • AHCYL1 antibody
    • DC expressed AHCY like molecule antibody
    • DC-expressed AHCY-like molecule antibody
    • DCAL antibody
    • Dendritic cell expressed AHCY like protein antibody
    • Inositol 1,4,5-trisphosphate receptor-binding protein antibody
    • IP3R binding protein released with inositol 1,4,5-trisphosphate antibody
    • IRBIT antibody
    • PRO0233 antibody
    • Putative adenosylhomocysteinase 2 antibody
    • S adenosyl L homocysteine hydrolase 2 antibody
    • S adenosylhomocysteine hydrolase like 1 antibody
    • S-adenosyl-L-homocysteine hydrolase 2 antibody
    • S-adenosylhomocysteine hydrolase-like protein 1 antibody
    • SAHH2_HUMAN antibody
    • XPVKONA antibody
    see all

Anti-AHCYL1 antibody images

  • Overlay histogram showing HeLa cells stained with ab56761 (red line). The cells were fixed with 80% methanol (5 min)/ and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56761, 0.01μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • AHCYL1 antibody (ab56761) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human stomach.


  • Predicted band size : 56 kDa
    AHCYL1 antibody (ab56761) at 1ug/lane + HeLa cell lysate at 25ug/lane.

References for Anti-AHCYL1 antibody (ab56761)

This product has been referenced in:
  • Jeong W  et al. Paradoxical expression of AHCYL1 affecting ovarian carcinogenesis between chickens and women. Exp Biol Med (Maywood) 237:758-67 (2012). Human, Chicken . Read more (PubMed: 22826361) »
  • Jeong W  et al. AHCYL1 is mediated by estrogen-induced ERK1/2 MAPK cell signaling and microRNA regulation to effect functional aspects of the avian oviduct. PLoS One 7:e49204 (2012). WB, ICC/IF, IHC-P ; Chicken . Read more (PubMed: 23145124) »

See all 2 Publications for this product

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