The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 56 kDa.
Use a concentration of 3 µg/ml.
Use 0.01-0.1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
Tissue specificityExpressed in dendritic cells.
PathwayAmino-acid biosynthesis; L-homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1.
Sequence similaritiesBelongs to the adenosylhomocysteinase family.
Developmental stageExpression increases markedly during activation of blood and skin DC (Langerhans cells), but is diminished in terminally differentiated tonsil DC.
DomainThe PEST region is essential for the interaction with ITPR1. The PDZ-binding region is required for maximal interaction with ITPR1 and is also responsible for the IP3-insensitive interaction with ITPR1.
Dendritic cell expressed AHCY like protein antibody
Inositol 1,4,5-trisphosphate receptor-binding protein antibody
IP3R binding protein released with inositol 1,4,5-trisphosphate antibody
Putative adenosylhomocysteinase 2 antibody
S adenosyl L homocysteine hydrolase 2 antibody
S adenosylhomocysteine hydrolase like 1 antibody
S-adenosyl-L-homocysteine hydrolase 2 antibody
S-adenosylhomocysteine hydrolase-like protein 1 antibody
Anti-AHCYL1 antibody images
Flow Cytometry - Anti-AHCYL1 antibody (ab56761)
Overlay histogram showing HeLa cells stained with ab56761 (red line). The cells were fixed with 80% methanol (5 min)/ and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56761, 0.01μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
AHCYL1 antibody (ab56761) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human stomach.
Western blot - AHCYL1 antibody (ab56761)
Predicted band size : 56 kDa AHCYL1 antibody (ab56761) at 1ug/lane + HeLa cell lysate at 25ug/lane.
References for Anti-AHCYL1 antibody (ab56761)
This product has been referenced in:
Jeong W et al. Paradoxical expression of AHCYL1 affecting ovarian carcinogenesis between chickens and women. Exp Biol Med (Maywood)237:758-67 (2012).
Read more (PubMed: 22826361) »
Jeong W et al. AHCYL1 is mediated by estrogen-induced ERK1/2 MAPK cell signaling and microRNA regulation to effect functional aspects of the avian oviduct. PLoS One7:e49204 (2012).
WB, ICC/IF, IHC-P
Read more (PubMed: 23145124) »