Overview

  • Product nameAnti-AHCYL1 antibody
    See all AHCYL1 primary antibodies
  • Description
    Mouse monoclonal to AHCYL1
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment: MSMPDAMPLP GVGEELKQAK EIEDAEKYSF MATVTKAPKK QIQFADDMQE FTKFPTKTGR RSLSRSISQS STDSYSSAAS YTDSSDDEVS PREKQQTNSK G, corresponding to amino acids 1-101 of human AHCYL1 (NP_006612) with a 26 kDa tag.

  • Positive control
    • HeLa and PC12 cell lysates; recombinant tagged fragment of human AHCYL1 (the immunogen).

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab77638 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 59 kDa.
ELISA Use at an assay dependent dilution.

Target

  • Tissue specificityExpressed in dendritic cells.
  • PathwayAmino-acid biosynthesis; L-homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1.
  • Sequence similaritiesBelongs to the adenosylhomocysteinase family.
  • Developmental stageExpression increases markedly during activation of blood and skin DC (Langerhans cells), but is diminished in terminally differentiated tonsil DC.
  • DomainThe PEST region is essential for the interaction with ITPR1. The PDZ-binding region is required for maximal interaction with ITPR1 and is also responsible for the IP3-insensitive interaction with ITPR1.
  • Cellular localizationEndoplasmic reticulum.
  • Information by UniProt
  • Database links
  • Alternative names
    • AA409031 antibody
    • AdoHcyase 2 antibody
    • Ahcy-rs3 antibody
    • AHCYL1 antibody
    • DC expressed AHCY like molecule antibody
    • DC-expressed AHCY-like molecule antibody
    • DCAL antibody
    • Dendritic cell expressed AHCY like protein antibody
    • Inositol 1,4,5-trisphosphate receptor-binding protein antibody
    • IP3R binding protein released with inositol 1,4,5-trisphosphate antibody
    • IRBIT antibody
    • PRO0233 antibody
    • Putative adenosylhomocysteinase 2 antibody
    • S adenosyl L homocysteine hydrolase 2 antibody
    • S adenosylhomocysteine hydrolase like 1 antibody
    • S-adenosyl-L-homocysteine hydrolase 2 antibody
    • S-adenosylhomocysteine hydrolase-like protein 1 antibody
    • SAHH2_HUMAN antibody
    • XPVKONA antibody
    see all

Anti-AHCYL1 antibody images

  • Anti-AHCYL1 antibody (ab77638) at 1/500 dilution + Recombinant fragment of human AHCYL1 at 0.2 µg

    Secondary
    Goat anti-Mouse IgG (H&L)-HRP at 1/5000 dilution

    Predicted band size : 59 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • Anti-AHCYL1 antibody (ab77638) at 1/500 dilution + HeLa cell lysate at 25 µg

    Secondary
    Goat anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 59 kDa
    Observed band size : 59 kDa
  • Anti-AHCYL1 antibody (ab77638) at 1/500 dilution + PC12 cell lysate at 25 µg

    Secondary
    Goat anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 59 kDa
    Observed band size : 59 kDa

References for Anti-AHCYL1 antibody (ab77638)

ab77638 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"