Anti-AHNAK antibody [EM-09] (ab68556)


  • Product name
    Anti-AHNAK antibody [EM-09]
    See all AHNAK primary antibodies
  • Description
    Mouse monoclonal [EM-09] to AHNAK
  • Host species
  • Tested applications
    Suitable for: WB, IP, ICC, Flow Cyt, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Does not react with: Rat
  • Immunogen

    Recombinant fragment corresponding to Human AHNAK (N terminal).

  • Positive control
    • HeLa human cervix carcinoma cell lysate and cells. Human primary fibroblasts.


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 15mM Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    >95% by SDS-PAGE
  • Purification notes
    ab68556 is purified from hybridoma culture supernatant by protein A affinity chromatography.
  • Clonality
  • Clone number
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab68556 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 629 kDa.
IP Use at an assay dependent concentration.
ICC 1/250. Permeabilization is required.
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


IHC-Fr Use at an assay dependent concentration.


  • Function
    May be required for neuronal cell differentiation.
  • Sequence similarities
    Contains 1 PDZ (DHR) domain.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • AHNAK antibody
    • AHNAK nucleoprotein (desmoyokin) antibody
    • AHNAK nucleoprotein antibody
    • AHNAKRS antibody
    • AHNK_HUMAN antibody
    • Desmoyokin antibody
    • Fragments antibody
    • MGC5395 antibody
    • Neuroblast differentiation associated protein AHNAK antibody
    • Neuroblast differentiation-associated protein AHNAK antibody
    • PM227 antibody
    see all


  • Human primary fibroblasts stained with ab68556 (green). Actin filaments were decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).
  • ab68556 at 1/250 dilution staining AHNAK (red) in human Hela cells by Immunocytochemistry/Immunofluorescence. Nuclei were stained with DAPI (blue).

  • Anti-AHNAK antibody [EM-09] (ab68556) at 2 µg/ml
    Performed under non-reducing conditions.

    Predicted band size: 629 kDa

    The material was 1% lauryl maltoside lysate of HeLa cells, non-reducing conditions, immunoprecipitated with another anti-AHNAK1 antibody. 4% stacking gel, 6% separating gel, U=190 V, 60 min. Blotting: 30 mA, 90 min. Detection antibody concentration: 2 microgram/ml. Please note the conditions were not optimalized.
  • Ab68556 (red) staining AHNAK in murine tongue by immunohistochemistry using frozen tissue sections. Actin filaments were decorated by phalloidin (green), cell nuclei stained with DAPI (blue).
  • Overlay histogram showing HeLa cells stained with ab68556 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab68556, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Hela cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.


ab68556 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

We do sell normal mouse serum, the catalogue number is ab7486 and ab138705.

The affinity purified antibody we recommend is ab139582;


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Thank you for following up.

I am happy to hear that the antibody is working as expected. Please do not hesitate to contact me if you have any additional questions. Good luck with your research!

Thank you for contacting Abcam regarding ab68556.

I have followed up with the lab and our shipping/receiving department and we have been unable to determine why this antibody is autofluorescing. I have confirmed that the antibody is not...

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Thank you for your patience. The lab sent the following explanation: The 150 kDa band seems to be a fragment arising due to manipulation with the extremely large AHNAK1 protein. Sometimes there are even more fragments observable, as the SDS PAGE ...

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Abcam has not validated the combination of species/application used in this Abreview.
Western blot
Rat Cell lysate - whole cell (adipocytes)
Loading amount
30 µg
Gel Running Conditions
Non-reduced Denaturing (4-12% gradient)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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Verified customer

Submitted Feb 01 2011


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