Overview

  • Product name
  • Description
    Rabbit polyclonal to AHRR
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Guinea pig, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 648-697 (PQSEPPHQLC ARGRGEQSCT CRAAEAAPVV KREPLDSPQW ATHSQGMVPG) of Human AHRR (NP_065782)

  • Positive control
    • HepG2 cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab85666 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 78 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Mediates dioxin toxicity and is involved in regulation of cell growth and differentiation. Represses the transcription activity of AHR by competing with this transcription factor for heterodimer formation with the ARNT and subsequently binding to the xenobiotic response element (XRE) sequence present in the promoter regulatory region of variety of genes. Represses CYP1A1 by binding the XRE sequence and recruiting ANKRA2, HDAC4 and/or HDAC5. Autoregulates its expression by associating with its own XRE site.
  • Tissue specificity
    Highly expressed in testis, lung, ovary, spleen and pancreas. Highly expressed in mononuclear cells (MNCs) from umbilical cord blood. Isoform 3 is highly expressed in lung, kidney, spleen and thymus. Down-regulated malignant tissue from different anatomical origins, including colon, breast, lung, stomach, cervix, and ovary.
  • Sequence similarities
    Contains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 PAS (PER-ARNT-SIM) domain.
  • Cellular localization
    Cytoplasm. Nucleus. Predominantly in the nuclear compartment. First cytoplasmic, translocates into the nuclear compartment upon interaction with ARNT in the cytoplasmic compartment.
  • Information by UniProt
  • Database links
  • Alternative names
    • AHH antibody
    • AhR repressor antibody
    • AhRR antibody
    • AHRR_HUMAN antibody
    • aryl hydrocarbon hydroxylase regulator antibody
    • aryl hydrocarbon receptor regulator antibody
    • Aryl hydrocarbon receptor repressor antibody
    • Arylhydrocarbon receptor repressor antibody
    • bHLHe77 antibody
    • Class E basic helix-loop-helix protein 77 antibody
    • Dioxin receptor repressor antibody
    • KIAA1234 antibody
    • MGC167813 antibody
    • MGC176630 antibody
    • OTTHUMP00000221488 antibody
    • OTTHUMP00000221489 antibody
    see all

Images

  • Anti-AHRR antibody (ab85666) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 78 kDa
    Observed band size : 92 kDa (why is the actual band size different from the predicted?)

References

This product has been referenced in:
  • Joo MS  et al. miR-125b transcriptionally increased by Nrf2 inhibits AhR repressor, which protects kidney from cisplatin-induced injury. Cell Death Dis 4:e899 (2013). Read more (PubMed: 24176857) »
  • To KK  et al. Upregulation of ABCG2 by Romidepsin via the Aryl Hydrocarbon Receptor Pathway. Mol Cancer Res 9:516-27 (2011). WB ; Human . Read more (PubMed: 21357443) »

See all 2 Publications for this product

Customer reviews and Q&As

Thank you for getting back to me and for passing some further information. I could offer either a new vial free of charge or a credit note which can be used for future purchase. Please could you get back to your troubled customer and discuss ho...

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Thank you for contacting Abcam Technical Team and for taking the time to provide some useful details of the experiments. I am very sorry to hear that your customer is having problems with this product. Though you have kindly provided some detail...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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