The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: 1/4000 (50%B).
ELISA: 1/200K (EPT).
WB: 1/100. Detects a band of approximately 56 kDa, a duplet+ 36 - 42 kDa band and a diffuse band of 27/29 kDa. MW from peptide sequence is 44 kD but AHSD is glycosylated.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionPromotes endocytosis, possesses opsonic properties and influences the mineral phase of bone. Shows affinity for calcium and barium ions.
Tissue specificitySynthesized in liver and selectively concentrated in bone matrix. Secreted in plasma. It is also found in dentin in much higher quantities than other plasma proteins.
Sequence similaritiesBelongs to the fetuin family. Contains 2 cystatin fetuin-A-type domains.
Post-translational modificationsPhosphorylation sites are present in the extracelllular medium.