Overview

  • Product name
  • Description
    Rabbit polyclonal to AHSG
  • Tested applications
    Suitable for: RIA, WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human Alpha 2 HS Glycoprotein

Properties

Applications

Our Abpromise guarantee covers the use of ab47979 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
RIA Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Predicted molecular weight: 40 kDa.
ELISA Use at an assay dependent dilution.
ICC/IF Use a concentration of 1 µg/ml.

Target

  • Function
    Promotes endocytosis, possesses opsonic properties and influences the mineral phase of bone. Shows affinity for calcium and barium ions.
  • Tissue specificity
    Synthesized in liver and selectively concentrated in bone matrix. Secreted in plasma. It is also found in dentin in much higher quantities than other plasma proteins.
  • Sequence similarities
    Belongs to the fetuin family.
    Contains 2 cystatin fetuin-A-type domains.
  • Post-translational
    modifications
    Phosphorylation sites are present in the extracelllular medium.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • 59 kDa bone sialic acid-containing protein antibody
    • A2HS antibody
    • Aa2-066 antibody
    • AHS antibody
    • Ahsg alpha-2-HS-glycoprotein antibody
    • Ahsg antibody
    • Alpha 2 HS Glycoprotein antibody
    • Alpha 2 Z globulin antibody
    • Alpha-2-HS-glycoprotein antibody
    • Alpha-2-HS-glycoprotein chain B antibody
    • Alpha-2-Z-globulin antibody
    • Asialofetuin antibody
    • Ba alpha 2 glycoprotein antibody
    • Ba-alpha-2-glycoprotein antibody
    • BSP antibody
    • Countertrypin antibody
    • Fetua antibody
    • FETUA_HUMAN antibody
    • Fetuin , mouse, homolog of antibody
    • Fetuin A antibody
    • Fetuin-A antibody
    • Glycoprotein PP63 antibody
    • HSGA antibody
    • pp63 antibody
    see all

Images

  • ICC/IF image of ab47979 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47979, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

ab47979 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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