Overview

  • Product name
  • Description
    Goat polyclonal to AICDA
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow, Dog
  • Immunogen

    Peptide with sequence

    RRKFLYQFKNVR

    -C, from the N terminus of human AICDA protein (NP_065712.1).

  • Positive control
    • Human tonsil lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77401 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    Peptide ELISA: Use at an assay dependent dilution. Detection limit dilution 1:1,000.
    WB: Use at a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 24 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      RNA-editing deaminase involved in somatic hypermutation, gene conversion, and class-switch recombination. Required for several crucial steps of B-cell terminal differentiation necessary for efficient antibody responses.
    • Tissue specificity
      Strongly expressed in lymph nodes and tonsils.
    • Involvement in disease
      Defects in AICDA are the cause of hyper-IgM immunodeficiency syndrome type 2 (HIGM2) [MIM:605258]; also known as hyper-IgM syndrome 2. HIGM2 is an autosomal recessive disorder characterized by normal or elevated serum IgM levels with absence of IgG, IgA, and IgE, resulting in a profound susceptibility to bacterial infections. HIGM2 causes the absence of Ig class switch recombination (CSR), the lack of Ig somatic hypermutations, and lymph node hyperplasia caused by the presence of giant germinal centers.
    • Sequence similarities
      Belongs to the cytidine and deoxycytidylate deaminase family.
    • Information by UniProt
    • Database links
    • Alternative names
      • Activation induced cytidine deaminase antibody
      • Activation induced deaminase antibody
      • Activation-induced cytidine deaminase antibody
      • AICDA antibody
      • AICDA_HUMAN antibody
      • AID antibody
      • ARP 2 antibody
      • ARP2 antibody
      • CDA 2 antibody
      • CDA2 antibody
      • Cytidine aminohydrolase antibody
      • HIGM2 antibody
      • Integrated into Burkitt's lymphoma cell line Ramos antibody
      see all

    Anti-AICDA antibody images

    • Anti-AICDA antibody (ab77401) at 0.3 µg/ml + Human tonsil lysate in RIPA buffer at 35 µg
      Developed using the ECL technique

      Predicted band size : 24 kDa
      Observed band size : 26 kDa (why is the actual band size different from the predicted?)


      Exposure time : 1 hour

    References for Anti-AICDA antibody (ab77401)

    ab77401 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab77401.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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