Overview

  • Product nameAnti-AICDA antibody - ChIP Grade
    See all AICDA primary antibodies
  • Description
    Rabbit polyclonal to AICDA - ChIP Grade
  • Tested applicationsWB, ChIPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide derived from residues 1 - 100 of Human AID.

    .

  • Positive control
    • This antibody gave a positive signal in AICDA 293T Cell Transient Overexpression Lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab5197 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).Can be blocked with Human AICDA peptide (ab13718).
ChIP Use at an assay dependent concentration.

Target

  • FunctionRNA-editing deaminase involved in somatic hypermutation, gene conversion, and class-switch recombination. Required for several crucial steps of B-cell terminal differentiation necessary for efficient antibody responses.
  • Tissue specificityStrongly expressed in lymph nodes and tonsils.
  • Involvement in diseaseDefects in AICDA are the cause of hyper-IgM immunodeficiency syndrome type 2 (HIGM2) [MIM:605258]; also known as hyper-IgM syndrome 2. HIGM2 is an autosomal recessive disorder characterized by normal or elevated serum IgM levels with absence of IgG, IgA, and IgE, resulting in a profound susceptibility to bacterial infections. HIGM2 causes the absence of Ig class switch recombination (CSR), the lack of Ig somatic hypermutations, and lymph node hyperplasia caused by the presence of giant germinal centers.
  • Sequence similaritiesBelongs to the cytidine and deoxycytidylate deaminase family.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activation induced cytidine deaminase antibody
    • Activation induced deaminase antibody
    • Activation-Induced Cytidine Deaminase antibody
    • AICD antibody
    • AICDA antibody
    • AICDA_HUMAN antibody
    • AID antibody
    • ARP 2 antibody
    • ARP2 antibody
    • CDA 2 antibody
    • CDA2 antibody
    • Cytidine aminohydrolase antibody
    • HIGM2 antibody
    • Integrated into Burkitt's lymphoma cell line Ramos antibody
    see all

Anti-AICDA antibody - ChIP Grade images

  • Anti-AICDA antibody - ChIP Grade (ab5197) at 1 µg/ml + AICDA 293T Cell Transient Overexpression Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Predicted band size : 24 kDa
    Observed band size : 24 kDa

References for Anti-AICDA antibody - ChIP Grade (ab5197)

This product has been referenced in:
  • Dayal S  et al. High resolution analysis of the chromatin landscape of the IgE switch region in human B cells. PLoS One 6:e24571 (2011). ChIP ; Human . Read more (PubMed: 21949728) »
  • Duquette ML  et al. AID binds to transcription-induced structures in c-MYC that map to regions associated with translocation and hypermutation. Oncogene 24:5791-8 (2005). Read more (PubMed: 15940261) »

See all 2 Publications for this product

Product Wall

DISCOUNT CODE: ***
Expiration date: February 13, 2013
Value: $337

This code will give you $337 off your next order before the expiration date. To redeem this offer, please submit an Abreview for ab5197 in flow cytometry and include ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application ChIP
Sample Human Cell lysate - other (Primary tonsillar B cells)
Specification Primary tonsillar B cells
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 4 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% Formaldehyde
Detection step Taqman
Positive control AID ChIP followed by qPCR analysis for known AID binding sites:
IgG switch region
IgM switch region
BIRC3 promoter
Negative control AID ChIP followed by qPCR analysis for known sites where AID is known not to bind:
Neuro D1 gene
Username

Dr. David Fear

Verified customer

Submitted Aug 13 2012

Thank you for contacting us this morning.

As discussed over the phone, I have generated a testing discount for anti-AICDA antibody (ab5197) for use in ChIP:

Discount code:

Expiration date:



This code wi...

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Thank you for the phone call and your patience.

The laboratory has now provided the images of the results with the new and old batch. The old batch has been tested by a collaborator and the new batch has been tested in the laboratory here - t...

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Thank you for your enquiry regarding ab5197 and ab13668. Having done a BLAST search (for gallus gallus) for the immunogen sequences used to generate those antibodies I found: 92% homology for AID : Query: 1 NVRWAKGRRETYLC 14 N+RW...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"