Overview

  • Product name
  • Description
    Rabbit polyclonal to AIFM3
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide (EGFSDRIVLC TLDRHLPYDR PKLSKSLDTQ PEQLALRPKE FFRAYGIEVL) corresponding to a region within internal sequence amino acids 216-265 of human AIFM3 (NP_653305).

  • Positive control
    • HeLa cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified by peptide affinity chromatography method.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab83791 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 67 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/312500.

Target

  • Function
    Induces apoptosis through a caspase dependent pathway. Reduces mitochondrial membrane potential.
  • Tissue specificity
    Ubiquitous. Expressed in bone marrow, cerebral cortex, liver, ovary, thymus, thyroid gland and tongue (at protein level).
  • Sequence similarities
    Belongs to the FAD-dependent oxidoreductase family.
    Contains 1 Rieske domain.
  • Domain
    The Rieske domain induces apoptosis.
  • Cellular localization
    Mitochondrion. Does not translocate to the nucleus upon induction of apoptosis.
  • Information by UniProt
  • Database links
  • Alternative names
    • AIFL antibody
    • Aifm3 antibody
    • AIFM3_HUMAN antibody
    • Apoptosis inducing factor 3 antibody
    • Apoptosis inducing factor like antibody
    • Apoptosis inducing factor like protein antibody
    • Apoptosis inducing factor, mitochondrion associated antibody
    • Apoptosis-inducing factor 3 antibody
    • Apoptosis-inducing factor-like protein antibody
    • FLJ30473 antibody
    • FLJ45137 antibody
    see all

Anti-AIFM3 antibody images

  • Anti-AIFM3 antibody (ab83791) at 1 µg/ml + HeLa cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 67 kDa
    Observed band size : 64 kDa (why is the actual band size different from the predicted?)

References for Anti-AIFM3 antibody (ab83791)

ab83791 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab83791.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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