Overview

  • Product name
  • Description
    Rabbit polyclonal to AIM2
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant fragment, corresponding to amino acids 93-341 of Human AIM2 (NM_004833).

  • Positive control
    • EC-109 and HepG2 cell lysates; Human fetal tonsil tissue

Properties

  • Form
    Lyophilised:Reconstitute with 200ul distilled sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab93015 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 39 kDa.
IHC-P 1/100 - 1/500.
ICC/IF Use at an assay dependent concentration.

Target

Anti-AIM2 antibody images

  • All lanes : Anti-AIM2 antibody (ab93015) at 1/500 dilution

    Lane 1 : EC-109 cell lysate
    Lane 2 : HepG2 cell lysate


    Predicted band size : 39 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of fetal spleen tissue sections labeling AIM2 with ab93015 at 1/100 dilution.

  • Immunocytochemistry/ Immunofluorescence analysis of Jurkat cells labeling AIM2 with ab93015 at 1/200 dilution. Cells were fixed with methanol and permeabilized with citrate buffer, pH 6.0. The cells were fixed with 4% BSA for 30 minutes at 25°C, followed by incubation with Anti-AIM2 antibody (ab93015) in blocking buffer for 16 hours at 4°C. A goat-anti-rabbit FITC secondary antibody was used at 1/100 dilution.

    See Abreview

  • Immunohistochemical staining of AIM2 in formalin fixed, paraffin embedded Human fetal tonsil showing nuclear staining with ab93015 at a dilution of 1/100.
  • ab93015 staining AIM2 in human monocytes (Apheresis) by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed, permeabilized, blocked with 2% BSA for 30 minutes at 25°C and then incubated with ab93015 at a 1/200 dilution for 2 hours at 25°C. The secondary used was an Alexa-Fluor 488 conjugated chicken anti-rabbit IgG (H+L) used at a 1/1000 dilution.

    See Abreview

References for Anti-AIM2 antibody (ab93015)

This product has been referenced in:
  • Ma X  et al. Loss of AIM2 expression promotes hepatocarcinoma progression through activation of mTOR-S6K1 pathway. Oncotarget 7:36185-36197 (2016). Read more (PubMed: 27167192) »
  • Zhen J  et al. AIM2 mediates inflammation-associated renal damage in hepatitis B virus-associated glomerulonephritis by regulating caspase-1, IL-1ß, and IL-18. Mediators Inflamm 2014:190860 (2014). WB, IHC-P ; Human . Read more (PubMed: 24701032) »

See all 8 Publications for this product

Product Wall

Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - whole cell (liver)
Specification
liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Nov 10 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - whole (skin)
Loading amount
30 µg
Specification
skin
Gel Running Conditions
Reduced Non-Denaturing (Native) (5-16% gel)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Feb 05 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Human Cultured Cells (Jurkat)
Specification
Jurkat
Fixative
Methanol
Permeabilization
Yes - citrate buffer, pH 6,0
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jan 29 2013

Thank you for your reply which has been forwarded to me as my colleague Anja is currently away from the office.

As requested, we are pleased to arrange a free of charge replacement. I can confirm that 1 vial of ab93015 has been added to your...

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Thank you for your email.

I do understand and appreciate the amount of time your customer hasspent it the laboratory. Our policy is that we are happy to offer a refund, credit note or free of charge replacement when a product is not working in...

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Application
Immunocytochemistry
Sample
Mouse Cultured Cells (RAW 264.7)
Specification
RAW 264.7
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton-X100 in 2% BSA for 15min
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Username

Dr. Mahesh Shivananjappa

Verified customer

Submitted May 19 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Human Cell (Monocytes from Apheresis)
Specification
Monocytes from Apheresis
Fixative
BD Cytofix/Cytoperm 20 min
Permeabilization
Yes - BD Cytofix/Cytoperm 20 min
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Dr. Mahesh Shivananjappa

Verified customer

Submitted Feb 01 2012

Thank you for contacting us.

I am sorry for the delay in gathering all the information that you have requested and getting back to you with it. I have contacted the suppliers of the products that you have enquired about and asked if the pe...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (lung)
Specification
lung
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6,0
Permeabilization
Yes - 0,1% Saponin / PBS
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jan 16 2012

Thank you for contacting us earlier, it was a pleasure speaking with you. I am sorry to hear that ab93015 is still giving you trouble.  I have set up a free of charge order for ab119845 for you, the order number is XXXXXX.  As I mentioned on the ph...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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