Overview

  • Product nameAnti-AIM2 antibody
    See all AIM2 primary antibodies
  • Description
    Rabbit polyclonal to AIM2
  • Tested applicationsSuitable for: WB, IHC-P, ICCmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant fragment, corresponding to amino acids 93-341 of Human AIM2 (NM_004833).

  • Positive control
    • EC-109 and HepG2 cell lysates; Human fetal tonsil tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab93015 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 39 kDa.
IHC-P 1/100 - 1/500.
ICC Use at an assay dependent concentration.

Target

Anti-AIM2 antibody images

  • Immunohistochemical staining of AIM2 in formalin fixed, paraffin embedded Human fetal tonsil showing nuclear staining with ab93015 at a dilution of 1/100.
  • All lanes : Anti-AIM2 antibody (ab93015) at 1/500 dilution

    Lane 1 : EC-109 cell lysate
    Lane 2 : HepG2 cell lysate


    Predicted band size : 39 kDa
  • ab93015 staining AIM2 in human monocytes (Apheresis) by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed, permeabilized, blocked with 2% BSA for 30 minutes at 25°C and then incubated with ab93015 at a 1/200 dilution for 2 hours at 25°C. The secondary used was an Alexa-Fluor 488 conjugated chicken anti-rabbit IgG (H+L) used at a 1/1000 dilution.

    See Abreview

References for Anti-AIM2 antibody (ab93015)

This product has been referenced in:
  • Zhen J  et al. AIM2 mediates inflammation-associated renal damage in hepatitis B virus-associated glomerulonephritis by regulating caspase-1, IL-1ß, and IL-18. Mediators Inflamm 2014:190860 (2014). WB, IHC-P ; Human . Read more (PubMed: 24701032) »
  • Chattergoon MA  et al. HIV and HCV activate the inflammasome in monocytes and macrophages via endosomal Toll-like receptors without induction of type 1 interferon. PLoS Pathog 10:e1004082 (2014). Read more (PubMed: 24788318) »

See all 6 Publications for this product

Product Wall

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Cell lysate - whole cell (liver)
Specification liver
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Abcam user community

Verified customer

Submitted Nov 10 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (skin)
Loading amount 30 µg
Specification skin
Gel Running Conditions Reduced Non-Denaturing (Native) (5-16% gel)
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

Submitted Feb 05 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Human Cultured Cells (Jurkat)
Specification Jurkat
Fixative Methanol
Permeabilization Yes - citrate buffer, pH 6,0
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jan 29 2013

Thank you for your reply which has been forwarded to me as my colleague Anja is currently away from the office.

As requested, we are pleased to arrange a free of charge replacement. I can confirm that 1 vial of ab93015 has been added to your...

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Thank you for your email.

I do understand and appreciate the amount of time your customer hasspent it the laboratory. Our policy is that we are happy to offer a refund, credit note or free of charge replacement when a product is not working in...

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Application Immunocytochemistry
Sample Mouse Cultured Cells (RAW 264.7)
Specification RAW 264.7
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton-X100 in 2% BSA for 15min
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Username

Dr. Mahesh Shivananjappa

Verified customer

Submitted May 19 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Human Cell (Monocytes from Apheresis)
Specification Monocytes from Apheresis
Fixative BD Cytofix/Cytoperm 20 min
Permeabilization Yes - BD Cytofix/Cytoperm 20 min
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Dr. Mahesh Shivananjappa

Verified customer

Submitted Feb 01 2012

Thank you for contacting us.

I am sorry for the delay in gathering all the information that you have requested and getting back to you with it. I have contacted the suppliers of the products that you have enquired about and asked if the pe...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (lung)
Specification lung
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6,0
Permeabilization Yes - 0,1% Saponin / PBS
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jan 16 2012

Thank you for contacting us earlier, it was a pleasure speaking with you. I am sorry to hear that ab93015 is still giving you trouble.  I have set up a free of charge order for ab119845 for you, the order number is XXXXXX.  As I mentioned on the ph...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"