Overview

  • Product nameAnti-AIMP3/p18 antibody
    See all AIMP3/p18 primary antibodies
  • Description
    Rabbit polyclonal to AIMP3/p18
  • Specificityab31543 reacts specifically with 21kDa p18 and immunoprecipitates with ATM/ATR
  • Tested applicationsWB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Native full length recombinant human p18

  • Positive control
    • HEK293 cell, U2OS cell and Hela cell

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.05% Sodium Azide
  • PurityWhole antiserum
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab31543 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/8000. Detects a band of approximately 21 kDa (predicted molecular weight: 18 kDa).
IP Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.

Target

  • FunctionPositive modulator of ATM response to DNA damage.
  • Tissue specificityDown-regulated in various cancer tissues.
  • Sequence similaritiesContains 1 GST C-terminal domain.
  • Cellular localizationCytoplasm. Nucleus. Cytoplasmic under growth arrest conditions. Translocated into the nucleus when growth resumes (S phase) and following DNA damage.
  • Information by UniProt
  • Database links
  • Alternative names
    • AIMP 3 antibody
    • AIMP3 antibody
    • Aminoacyl tRNA synthetase complex interacting multifunctional protein 3 antibody
    • Aminoacyl tRNA synthetase complex-interacting multifunctional protein 3 antibody
    • ARS interacting multifunctional protein 3 antibody
    • Cyclin dependent kinase 6 inhibitor antibody
    • EEF1E1 antibody
    • Elongation factor p18 antibody
    • Eukaryotic translation elongation factor 1 epsilon 1 antibody
    • Eukaryotic translation elongation factor 1 epsilon-1 antibody
    • Homolog of rat elongation factor p18 antibody
    • MCA3_HUMAN antibody
    • Multisynthase complex auxiliary component p18 antibody
    • Multisynthetase complex auxiliary component p18 antibody
    • p18 antibody
    • p18 component of aminoacyl tRNA synthetase complex antibody
    • p18 INK4c antibody
    • p18 NK6 antibody
    see all

Anti-AIMP3/p18 antibody images

  • Anti-AIMP3/p18 antibody (ab31543) at 1/20000 dilution + HEK293 cell line

    Predicted band size : 18 kDa
    Observed band size : 21 kDa (why is the actual band size different from the predicted?)
  • Anti-AIMP3/p18 antibody (ab31543) at 1/8000 dilution + whole cell lysate prepared from HEK293 cells at 50 µg/ml

    Secondary
    Goat anti-rabbit IgG (H+L) at 1/10000 dilution

    Predicted band size : 18 kDa
    Observed band size : 20 kDa (why is the actual band size different from the predicted?)

References for Anti-AIMP3/p18 antibody (ab31543)

This product has been referenced in:
  • Gulati P  et al. Role for the obesity-related FTO gene in the cellular sensing of amino acids. Proc Natl Acad Sci U S A 110:2557-62 (2013). WB ; Mouse . Read more (PubMed: 23359686) »
  • Du Y  et al. Elevation of Highly Up-regulated in Liver Cancer (HULC) by Hepatitis B Virus X Protein Promotes Hepatoma Cell Proliferation via Down-regulating p18. J Biol Chem 287:26302-11 (2012). WB ; Human . Read more (PubMed: 22685290) »

See all 4 Publications for this product

Product Wall

Thank you for contacting us and reporting the problems observed with your current batch of ab31543. As discussed over the phone, it may be that unfortunately you have received a faulty batch. I have organised for a free of charge replacement to be...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"