Overview

  • Product name
  • Description
    Rabbit polyclonal to AIMP3/p18
  • Specificity
    ab31543 reacts specifically with 21kDa p18 and immunoprecipitates with ATM/ATR
  • Tested applications
    Suitable for: WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Native full length recombinant human p18

  • Positive control
    • HEK293 cell, U2OS cell and Hela cell

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.05% Sodium Azide
  • Purity
    Whole antiserum
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab31543 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/8000. Detects a band of approximately 21 kDa (predicted molecular weight: 18 kDa).
IP Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.

Target

  • Function
    Positive modulator of ATM response to DNA damage.
  • Tissue specificity
    Down-regulated in various cancer tissues.
  • Sequence similarities
    Contains 1 GST C-terminal domain.
  • Cellular localization
    Cytoplasm. Nucleus. Cytoplasmic under growth arrest conditions. Translocated into the nucleus when growth resumes (S phase) and following DNA damage.
  • Information by UniProt
  • Database links
  • Alternative names
    • AIMP 3 antibody
    • AIMP3 antibody
    • Aminoacyl tRNA synthetase complex interacting multifunctional protein 3 antibody
    • Aminoacyl tRNA synthetase complex-interacting multifunctional protein 3 antibody
    • ARS interacting multifunctional protein 3 antibody
    • EEF1E1 antibody
    • Elongation factor p18 antibody
    • Eukaryotic translation elongation factor 1 epsilon 1 antibody
    • Eukaryotic translation elongation factor 1 epsilon-1 antibody
    • Homolog of rat elongation factor p18 antibody
    • MCA3_HUMAN antibody
    • Multisynthase complex auxiliary component p18 antibody
    • Multisynthetase complex auxiliary component p18 antibody
    • p18 antibody
    • p18 component of aminoacyl tRNA synthetase complex antibody
    see all

Images

  • Anti-AIMP3/p18 antibody (ab31543) at 1/20000 dilution + HEK293 cell line

    Predicted band size : 18 kDa
    Observed band size : 21 kDa (why is the actual band size different from the predicted?)
  • Anti-AIMP3/p18 antibody (ab31543) at 1/8000 dilution + whole cell lysate prepared from HEK293 cells at 50 µg/ml

    Secondary
    Goat anti-rabbit IgG (H+L) at 1/10000 dilution

    Predicted band size : 18 kDa
    Observed band size : 20 kDa (why is the actual band size different from the predicted?)

References

This product has been referenced in:
  • Gulati P  et al. Role for the obesity-related FTO gene in the cellular sensing of amino acids. Proc Natl Acad Sci U S A 110:2557-62 (2013). WB ; Mouse . Read more (PubMed: 23359686) »
  • Du Y  et al. Elevation of Highly Up-regulated in Liver Cancer (HULC) by Hepatitis B Virus X Protein Promotes Hepatoma Cell Proliferation via Down-regulating p18. J Biol Chem 287:26302-11 (2012). WB ; Human . Read more (PubMed: 22685290) »

See all 4 Publications for this product

Customer reviews and Q&As

Thank you for contacting us and reporting the problems observed with your current batch of ab31543. As discussed over the phone, it may be that unfortunately you have received a faulty batch. I have organised for a free of charge replacement to be...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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