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C-KDVDLSQPRKGRKP, corresponding to internal sequence amino acids 102-115 of Human AIRE (NP_000374.1).
Our Abpromise guarantee covers the use of ab111925 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use a concentration of 2.5 µg/ml.|
|WB||Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 58 kDa).|
|IHC-P||Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alkaline Phosphatase (AP) staining was used.|
ab111925 has not yet been referenced specifically in any publications.