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Synthetic peptide: QSMARPAAPFPS, corresponding to C terminal amino acids 534-545 of Human AIRE.
Our Abpromise guarantee covers the use of ab13573 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ChIP||Use at an assay dependent concentration.|
|WB||Use a concentration of 0.5 - 1.5 µg/ml. Can be blocked with Human AIRE peptide (ab23217).|
|IHC-P||Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
ab13573 (2µg/ml) staining of paraffin embedded Human Thymus shows staining of select nuclei following steamed antigen retrieval with Tris/EDTA buffer pH 9 and HRP-staining.
ab13573 (2µg/ml) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.