Overview

  • Product nameAnti-Ajuba antibody
    See all Ajuba primary antibodies
  • Description
    Rabbit polyclonal to Ajuba
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human Ajuba.

    (Peptide available as ab93791.)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: HepG2; MEL-1; WI38.

Properties

Applications

Our Abpromise guarantee covers the use of ab64451 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at a concentration of 1 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceAjuba is a member of LIM domain containing proteins that contribute to cell fate determination and regulate cell proliferation and differentiation. Ajuba is also involved in the regulation of actin cytoskeleton dynamics and cell migration. Ajuba plays a important role in the regulation of the kinase activity of AURKA/Aurora A for mitotic commitment. Is also a component of the IL 1 signaling pathway modulating IL 1 induced NF kappa B activation by influencing the assembly and activity of the PRKCZ/SQSTM1/TRAF6 multiprotein signaling complex. Ajuba is also a negative regulator of the Wnt signaling pathway.
    • Cellular localizationCytoplasm, cytoskeleton. Cell membrane. Cell junction. Nucleus. Centrosome. Note: Shuttles between the cytoplasm and the nucleus.
    • Database links
    • Alternative names
      • Ajuba LIM protein antibody
      • Ajuba, mouse, homolog of antibody
      • Jub ajuba homolog antibody
      • Jub antibody
      • LIM domain containing protein ajuba antibody
      • MGC15563 antibody
      • Protein ajuba antibody
      see all

    Anti-Ajuba antibody images

    • All lanes : Anti-Ajuba antibody (ab64451) at 1 µg/ml

      Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
      Lane 2 : MEL-1 (Human embryonic stem cell, male cell line) Whole Cell Lysate (ab27198)
      Lane 3 : WI-38 whole cell lysate (ab3960)

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 56 kDa
      Observed band size : 56 kDa
      Additional bands at : 23 kDa,48 kDa,73 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 10 minutes

    References for Anti-Ajuba antibody (ab64451)

    This product has been referenced in:
    • Kalan S  et al. LIM Protein Ajuba Participates in the Repression of the ATR-Mediated DNA Damage Response. Front Genet 4:95 (2013). Human . Read more (PubMed: 23755068) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"