Overview

  • Product nameAnti-AK2 antibody
    See all AK2 primary antibodies
  • Description
    Rabbit polyclonal to AK2
  • Tested applicationsSuitable for: WB, IHC-P, IHC-Fr, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human
  • Immunogen

    Synthetic peptide corresponding to Human AK2 aa 1-30 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).

  • Positive control
    • WB: CEM cell lysate and mouse kidney tissue lysate. IHC-P: human hepatocarcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab37594 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
IHC-P 1/50 - 1/100.
IHC-Fr Use at an assay dependent concentration. PubMed: 19043416
ICC/IF 1/100.

Target

  • FunctionCatalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. This small ubiquitous enzyme involved in energy metabolism and nucleotide synthesis that is essential for maintenance and cell growth. Plays a key role in hematopoiesis.
  • Tissue specificityPresent in most tissues. Present at high level in heart, liver and kidney, and at low level in brain, skeletal muscle and skin. Present in thrombocytes but not in erythrocytes, which lack mitochondria. Present in all nucleated cell populations from blood, while AK1 is mostly absent. In spleen and lymph nodes, mononuclear cells lack AK1, whereas AK2 is readily detectable. These results indicate that leukocytes may be susceptible to defects caused by the lack of AK2, as they do not express AK1 in sufficient amounts to compensate for the AK2 functional deficits (at protein level).
  • Involvement in diseaseDefects in AK2 are the cause of reticular dysgenesis (RDYS) [MIM:267500]; also known as aleukocytosis. RDYS is the most severe form of inborn severe combined immunodeficiencies (SCID) and is characterized by absence of granulocytes and almost complete deficiency of lymphocytes in peripheral blood, hypoplasia of the thymus and secondary lymphoid organs, and lack of innate and adaptive humoral and cellular immune functions, leading to fatal septicemia within days after birth. In bone marrow of individuals with reticular dysgenesis, myeloid differentiation is blocked at the promyelocytic stage, whereas erythro- and megakaryocytic maturation is generally normal.In addition, affected newborns have bilateral sensorineural deafness. Defects may be due to its absence in leukocytes and inner ear, in which its absence can not be compensated by AK1.
  • Sequence similaritiesBelongs to the adenylate kinase family. AK2 subfamily.
  • Cellular localizationMitochondrion intermembrane space.
  • Information by UniProt
  • Database links
  • Alternative names
    • Adenylate kinase 2 antibody
    • adenylate kinase 2, mitochondrial antibody
    • Adenylate kinase isoenzyme 2 antibody
    • ADK2 antibody
    • AK 2 antibody
    • ak2 antibody
    • ATP AMP transphosphorylase antibody
    • ATP-AMP transphosphorylase 2 antibody
    • EC 2.7.4.3 antibody
    • KAD2_HUMAN antibody
    • mitochondrial antibody
    see all

Anti-AK2 antibody images

  • Anti-AK2 antibody (ab37594) + CEM cell lysate at 35 µg

    Predicted band size : 26 kDa
  • ab37594 at 1/100 dilution detecting AK2 in inner ear (cochlea)-stria vascularis cells from mouse. AK2 (dark blue) is specifically expressed in the lumen of the capillaries and terminal blood vessels (stained green with isolectin) of the stria vascularis in the mouse cochlea (at postnatal day 7 (PN7)). Blood vessels in the spiral ligament do not contain AK2. Blood vessels in the spiral ligament do not contain AK2. Cell nuclei are stained in light blue by Hoechst staining. Actin is stained using TRITC conjugated phalloidin.

    See Abreview

  • Immunohistochemistry (Formalin/PFa-fixed paraffin-embedded sections) analysis of human hepatocarcinoma (HC) tissue labelling AK2 with ab37594 at 1/50. A peroxidase-conjugated anti-rabbit IgG was used as the secondary antibody, followed by AEC staining.

  • Anti-AK2 antibody (ab37594) at 1/100 dilution + Mouse kidney tissue lysate.

    Predicted band size : 26 kDa
    Observed band size : 26 kDa
    Additional bands at : 55 kDa,60 kDa,75 kDa. We are unsure as to the identity of these extra bands.

References for Anti-AK2 antibody (ab37594)

This product has been referenced in:
  • Wilson CH  et al. Identifying natural substrates for dipeptidyl peptidases 8 and 9 using terminal amine isotopic labeling of substrates (TAILS) reveals in vivo roles in cellular homeostasis and energy metabolism. J Biol Chem 288:13936-49 (2013). WB ; Human . Read more (PubMed: 23519473) »
  • Lagresle-Peyrou C  et al. Human adenylate kinase 2 deficiency causes a profound hematopoietic defect associated with sensorineural deafness. Nat Genet 41:106-11 (2009). IHC-Fr ; Mouse . Read more (PubMed: 19043416) »

See all 5 Publications for this product

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The Anti-AK2 antibody (ab37594) is provided as a protein G purified fraction with a concentration of 0.25 mg/mL.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (inner ear (cochlea) - stria vascularis.)
Specification inner ear (cochlea) - stria vascularis.
Fixative Paraformaldehyde
Permeabilization Yes - triton
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 20°C
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Dr. Vincent Michel

Verified customer

Submitted Apr 09 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"