Anti-AK2 antibody [EPR11388(B)] (ab166901)


  • Product name
    Anti-AK2 antibody [EPR11388(B)]
    See all AK2 primary antibodies
  • Description
    Rabbit monoclonal [EPR11388(B)] to AK2
  • Host species
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human AK2.

  • Positive control
    • Fetal kidney, Fetal liver, HepG2, MCF-7 and HeLa lysates, paraffin-embedded Human kidney tissue, paraffin-embedded Human stomach tissue,
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab166901 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 26 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/500.

For unpurified use at 1/100 - 1/250.

IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function
      Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. This small ubiquitous enzyme involved in energy metabolism and nucleotide synthesis that is essential for maintenance and cell growth. Plays a key role in hematopoiesis.
    • Tissue specificity
      Present in most tissues. Present at high level in heart, liver and kidney, and at low level in brain, skeletal muscle and skin. Present in thrombocytes but not in erythrocytes, which lack mitochondria. Present in all nucleated cell populations from blood, while AK1 is mostly absent. In spleen and lymph nodes, mononuclear cells lack AK1, whereas AK2 is readily detectable. These results indicate that leukocytes may be susceptible to defects caused by the lack of AK2, as they do not express AK1 in sufficient amounts to compensate for the AK2 functional deficits (at protein level).
    • Involvement in disease
      Defects in AK2 are the cause of reticular dysgenesis (RDYS) [MIM:267500]; also known as aleukocytosis. RDYS is the most severe form of inborn severe combined immunodeficiencies (SCID) and is characterized by absence of granulocytes and almost complete deficiency of lymphocytes in peripheral blood, hypoplasia of the thymus and secondary lymphoid organs, and lack of innate and adaptive humoral and cellular immune functions, leading to fatal septicemia within days after birth. In bone marrow of individuals with reticular dysgenesis, myeloid differentiation is blocked at the promyelocytic stage, whereas erythro- and megakaryocytic maturation is generally normal.In addition, affected newborns have bilateral sensorineural deafness. Defects may be due to its absence in leukocytes and inner ear, in which its absence can not be compensated by AK1.
    • Sequence similarities
      Belongs to the adenylate kinase family. AK2 subfamily.
    • Cellular localization
      Mitochondrion intermembrane space.
    • Information by UniProt
    • Database links
    • Alternative names
      • Adenylate kinase 2 antibody
      • adenylate kinase 2, mitochondrial antibody
      • Adenylate kinase isoenzyme 2 antibody
      • ADK2 antibody
      • AK 2 antibody
      • ak2 antibody
      • ATP AMP transphosphorylase antibody
      • ATP-AMP transphosphorylase 2 antibody
      • EC antibody
      • KAD2_HUMAN antibody
      • mitochondrial antibody
      see all


    • All lanes : Anti-AK2 antibody [EPR11388(B)] (ab166901) at 1/1000 dilution

      Lane 1 : Fetal kidney lysate
      Lane 2 : Fetal liver lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      All lanes : goat anti-rabbit HRP antibody at 1/2000 dilution

      Predicted band size: 26 kDa

    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling AK2 using ab166901 at 1/100 dilution.
    • Immunocytochemistry/Immunofluorescence analysis MCF-7 (human breast carcinoma) cells labelling AK2 with purified ab166901 at a dilution of 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (ab150077) at dilution of 1/1000 was used as the secondary antibody. Nuclei counterstained with DAPI (blue).

      Secondary Only Control: PBS was used instead of the primary antibody as the negative control.

    • Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling AK2 using ab166901 at 1/100 dilution.
    • Immunoprecipitation of AK2 from HeLa cell lysate pellet using ab166901 at 1/10 dilution followed by immunoblotting. HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.


    ab166901 has not yet been referenced specifically in any publications.

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