Overview

  • Product name
  • Description
    Rabbit polyclonal to AK3L1
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Cow
  • Immunogen

    Recombinant protein fragment corresponding to a region within amino acids 1 and 197 of Human AK3L1 (UniprotID: P27144).

  • Positive control
    • Human colon carcinoma tissue; rat RT2 xenograft tissue; HeLa, HepG2 and HCT116 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab152162 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 25 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval also possible using Tris-EDTA buffer (pH8.0).

Target

Images

  • All lanes : Anti-AK3L1 antibody (ab152162) at 1/1000 dilution

    Lane 1 : HeLa whole cell lysate
    Lane 2 : HepG2 whole cell lysate
    Lane 3 : HCT116 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 25 kDa
  • Immunohistochemical analysis of paraffin-embedded rat RT2 xenograft tissue, staining AK3L1 using ab152162 at a 1/500 dilution.
  • Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue, staining AK3L1 using ab152162 at a 1/500 dilution.

References

ab152162 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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