Overview

  • Product nameAnti-AKAP1 antibody
    See all AKAP1 primary antibodies
  • Description
    Rabbit polyclonal to AKAP1
  • Tested applicationsSuitable for: WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthesized peptide derived from an internal region of human AKAP1.

  • Positive control
    • IHC-P: Human brain tissue WB: Extracts from HUVEC cells and COLO cells ICC/IF: COS7 cells

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab69580 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000.
ELISA 1/5000.
IHC-P 1/50 - 1/100.
ICC/IF 1/500 - 1/1000.

Target

  • FunctionBinds to type I and II regulatory subunits of protein kinase A and anchors them to the cytoplasmic face of the mitochondrial outer membrane.
  • Tissue specificityAKAP149 is highly expressed in prostate and small intestine whereas S-AKAP84 is expressed in kidney, pancreas, liver, lung and brain. AKAP149 is also expressed in colon carcinoma.
  • Sequence similaritiesContains 1 KH domain.
    Contains 1 Tudor domain.
  • DomainRII-alpha binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer.
  • Cellular localizationMitochondrion outer membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • A kinase anchor protein antibody
    • A-kinase anchor protein 1 antibody
    • A-kinase anchor protein 149 kDa antibody
    • AKAP 149 antibody
    • AKAP antibody
    • Akap1 antibody
    • AKAP1_HUMAN antibody
    • D-AKAP-1 antibody
    • DAKAP1 antibody
    • Dual specificity A-kinase-anchoring protein 1 antibody
    • mitochondrial antibody
    • PRKA1 antibody
    • Protein kinase A-anchoring protein 1 antibody
    • Protein kinase A1 antibody
    • S-AKAP84 antibody
    • SAKAP84 antibody
    • Spermatid A-kinase anchor protein 84 antibody
    see all

Anti-AKAP1 antibody images

  • All lanes : Anti-AKAP1 antibody (ab69580) at 1/500 dilution

    Lane 1 : extracts from COLO cells without immunising peptide
    Lane 2 : extracts from HUVEC cells without immunising peptide
    Lane 3 : extracts from COLO cells with immunising peptide

    Lysates/proteins at 30 µg per lane.


    Observed band size : 92 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of AKAP1 in paraffin embedded human brain tissue using 1/50 ab69580. Left image: untreated sample. Right image: sample treated with immunising peptide.
  • Immunofluorescence analysis of AKAP1 in COS7 cells, using 1/500 ab69580. Left image: untreated cells. Right image: cells treated with immunising peptide.

References for Anti-AKAP1 antibody (ab69580)

ab69580 has not yet been referenced specifically in any publications.

Product Wall

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The immunogen sequence is from 306aa to 345aa. Swissrot No.: Q92667.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"