Overview

  • Product nameAnti-AKAP10 antibody
    See all AKAP10 primary antibodies
  • Description
    Rabbit polyclonal to AKAP10
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 600-662 (ESEPEPDVRK SKGSMFSQAM KKWVQGNTDE AQEELAWKIA KMIVSDIMQQ AQYDQPLEKS TKL) of Human AKAP10

  • Positive control
    • HeLa, HepG2 cells. OVCAR3 tissue, T(gastric CA) tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97354 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 74 kDa.
IHC-P 1/100 - 1/250.

Target

  • FunctionDifferentially targeted protein that binds to type I and II regulatory subunits of protein kinase A and anchors them to the mitochondria or the plasma membrane. Although the physiological relevance between PKA and AKAPS with mitochondria is not fully understood, one idea is that BAD, a proapoptotic member, is phosphorylated and inactivated by mitochondria-anchored PKA. It cannot be excluded too that it may facilitate PKA as well as G protein signal transduction, by acting as an adapter for assembling multiprotein complexes. With its RGS domain, it could lead to the interaction to G-alpha proteins, providing a link between the signaling machinery and the downstream kinase.
  • Involvement in diseaseGenetic variations in AKAP10 are a cause of susceptibility to sudden cardiac death (SCD) [MIM:115080]. Unexpected rapid natural death due to cardiovascular collapse within one hour of initial symptoms. It is usually caused by the worsening of existing heart diseases. The sudden onset of symptoms, such as chest pain and cardiac arrhythmias, particularly ventricular tachycardia, can lead to the loss of consciousness and cardiac arrest followed by biological death. Note=Increased susceptibility to sudden cardiac death may be conferred by AKAP10 variants that are associated with markers of low vagus nerve sensitivity, e.g. fast basal heart rate and low heart rate variability.
  • Sequence similaritiesContains 2 RGS domains.
  • DomainRII-alpha binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer.
  • Cellular localizationMitochondrion. Membrane. Cytoplasm. Predominantly mitochondrial but also membrane associated and cytoplasmic.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1500031L16Rik antibody
    • A kinase (PRKA) anchor protein 10 antibody
    • A kinase anchor protein 10 antibody
    • A kinase anchor protein 10 mitochondrial antibody
    • A-kinase anchor protein 10 antibody
    • AKA10_HUMAN antibody
    • akap 10 antibody
    • AKAP-10 antibody
    • AKAP10 antibody
    • B130049N18Rik antibody
    • D AKAP 2 antibody
    • D-AKAP-2 antibody
    • dakap 2 antibody
    • dakap2 antibody
    • Dual specific A kinase anchoring protein 2 antibody
    • Dual specificity A kinase-anchoring protein 2 antibody
    • mgc9410 antibody
    • MGC9414| antibody
    • Mitochondrial A kinase PPKA anchor protein 10 antibody
    • mitochondrial antibody
    • prka 10 antibody
    • PRKA10 antibody
    • Protein A kinase anchoring protein 10 antibody
    • Protein A kinase anchoring protein 10 mitochondrial precursor antibody
    • Protein kinase A anchoring protein 10 antibody
    • Protein kinase A-anchoring protein 10 antibody
    see all

Anti-AKAP10 antibody images

  • Anti-AKAP10 antibody (ab97354) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size : 74 kDa
  • Immunohistochemical analysis of paraffin-embedded T(gastric CA), using AKAP10 antibody (ab97354) at 1/100 dilution.
  • Immunohistochemical analysis of paraffin-embedded OVCAR3, using AKAP10 antibody (ab97354) at 1/100 dilution.

References for Anti-AKAP10 antibody (ab97354)

ab97354 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"