Overview

  • Product name
    Anti-AKAP11 antibody
  • Description
    Rabbit polyclonal to AKAP11
  • Tested applications
    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence, corresponding to amino acids 1190-1281 of Human AKAP11 (UniProt ID: Q9UKA4).

  • Positive control
    • Human kidney tissue

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122529 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.
IHC-P 1/1000. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.

Target

  • Function
    Binds to type II regulatory subunits of protein kinase A and anchors/targets them.
  • Tissue specificity
    Expressed in heart, brain, lung, liver, kidney, testis and ovary. Weakly expressed in skeletal muscle, pancreas and spleen.
  • Sequence similarities
    Belongs to the AKAP110 family.
  • Domain
    RII-alpha binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer.
  • Cellular localization
    Cytoplasm. Cytoplasm > cytoskeleton > centrosome. Cytoplasmic in premeiotic pachytene spermatocytes and in the centrosome of developing postmeiotic germ cells, while a midpiece/centrosome localization was found in elongating spermatocytes and mature sperm.
  • Information by UniProt
  • Database links
  • Alternative names
    • A-kinase anchor protein 11 antibody
    • A-kinase anchor protein 220 kDa antibody
    • AKA11_HUMAN antibody
    • AKAP 220 antibody
    • AKAP-11 antibody
    • AKAP11 antibody
    • hAKAP220 antibody
    • PRKA11 antibody
    • Protein kinase A-anchoring protein 11 antibody
    see all

Images

  • Immunohistochemical staining of AKAP11 in paraffin embedded Human kidney tissue by Immunohistochemistry, using ab122529 at a 1/1000 dilution. The image shows strong cytoplasmic positivity in cells in tubules.

References

ab122529 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab122529.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up