The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 4 µg/ml.
1/1000. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.
FunctionBinds to type II regulatory subunits of protein kinase A and anchors/targets them.
Tissue specificityExpressed in heart, brain, lung, liver, kidney, testis and ovary. Weakly expressed in skeletal muscle, pancreas and spleen.
Sequence similaritiesBelongs to the AKAP110 family.
DomainRII-alpha binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer.
Cellular localizationCytoplasm. Cytoplasm > cytoskeleton > centrosome. Cytoplasmic in premeiotic pachytene spermatocytes and in the centrosome of developing postmeiotic germ cells, while a midpiece/centrosome localization was found in elongating spermatocytes and mature sperm.
Immunohistochemical staining of AKAP11 in paraffin embedded Human kidney tissue by Immunohistochemistry, using ab122529 at a 1/1000 dilution. The image shows strong cytoplasmic positivity in cells in tubules.
References for Anti-AKAP11 antibody (ab122529)
has not yet been referenced specifically in any publications.
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