Overview

  • Product nameAnti-AKAP12 antibody
    See all AKAP12 primary antibodies
  • Description
    Sheep polyclonal to AKAP12
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Full length native protein (purified) (Rat).

  • General notesWorking dilution samples should be discarded if not used within 12 hours.


    AKAP12 is a major protein kinase C substrate with tumor suppressor activity in models of src- and ras-induced oncogenic transformation. AKAP12 regulates actin-based cytoskeletal and tissue architecture, as well as kinases such as protein kinase C (PKC), protein kinase A (PKA) and calmodulin. Additionally, AKAP12 has been implicated in the formation of migratory processes and cell migration during embryogenesis. Rat AKAP12 shares extensive homology with human Gravin, an autoantigen in myasthenia gravis that encodes kinase scaffolding functions and whose expression pattern in fibroblasts and nerves suggests a role in cell motility.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.08% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein A purified
  • Primary antibody notesAKAP12 is a major protein kinase C substrate with tumor suppressor activity in models of src- and ras-induced oncogenic transformation. AKAP12 regulates actin-based cytoskeletal and tissue architecture, as well as kinases such as protein kinase C (PKC), protein kinase A (PKA) and calmodulin. Additionally, AKAP12 has been implicated in the formation of migratory processes and cell migration during embryogenesis. Rat AKAP12 shares extensive homology with human Gravin, an autoantigen in myasthenia gravis that encodes kinase scaffolding functions and whose expression pattern in fibroblasts and nerves suggests a role in cell motility.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab10346 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 - 5 µg/ml. Predicted molecular weight: 172 kDa.
IP Use a concentration of 2 - 5 µg/ml.
IHC-P 1/800. PubMed: 21334414

Target

  • FunctionAnchoring protein that mediates the subcellular compartmentation of protein kinase A (PKA) and protein kinase C (PKC).
  • Tissue specificityExpressed in endothelial cells, cultured fibroblasts and osteosarcoma, but not in platelets, leukocytes, monocytic cell lines or peripherical blood cells.
  • Sequence similaritiesContains 3 AKAP domains.
  • DomainPolybasic regions located between residues 266 and 557 are involved in binding PKC.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationCytoplasm > cell cortex. Cytoplasm > cytoskeleton. May be part of the cortical cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • A kinase (PRKA) anchor protein (gravin) 12 antibody
    • A kinase Anchor Protein 12 antibody
    • A kinase anchor protein 250kDa antibody
    • A-kinase anchor protein 12 antibody
    • A-kinase anchor protein 250 kDa antibody
    • AKA12_HUMAN antibody
    • AKAP 12 antibody
    • AKAP 250 antibody
    • AKAP-12 antibody
    • Akap12 antibody
    • AKAP250 antibody
    • DKFZp686M0430 antibody
    • DKFZp686O0331 antibody
    • FLJ20945 antibody
    • FLJ97621 antibody
    • Gercelin antibody
    • Gravin antibody
    • Kinase scaffold protein gravin antibody
    • Myasthenia gravis autoantigen antibody
    • Myasthenia gravis autoantigen gravin antibody
    • OTTHUMP00000017426 antibody
    • OTTHUMP00000017427 antibody
    • Src-Suppressed C Kinase Substrate antibody
    • Srcs5 antibody
    • SSeCKS antibody
    • Tsga12 antibody
    see all

Anti-AKAP12 antibody images

  • ab10346 staining AKAP12 in Rat brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 4% paraformaldehyde. Samples were incubated with primary antibody (1/800) overnight at 4°C. 0 hours (left), 6 hours (middle), 1 day (right) after middle cerebral artery occlusion. Scale bar = 20µm.

References for Anti-AKAP12 antibody (ab10346)

This product has been referenced in:
  • Zan L  et al. Temporal profile of Src, SSeCKS, and angiogenic factors after focal cerebral ischemia: correlations with angiogenesis and cerebral edema. Neurochem Int 58:872-9 (2011). WB, IHC-P ; Rat . Read more (PubMed: 21334414) »

See 1 Publication for this product

Product Wall

Thank you for your enquiry regarding ab10356. The references on the datasheet are general background references and indeed have not used ab10356. We currently are not aware of publications which have used this antibody. We have added the predicted mo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"