• Product name
    Anti-AKAP12 antibody [JP74]
    See all AKAP12 primary antibodies
  • Description
    Mouse monoclonal [JP74] to AKAP12
  • Host species
  • Tested applications
    Suitable for: WB, IP, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Tissue/ cell preparation (Human) - fractionated membrane cortex proteins from neuron cell lines NT2-N and SK-N-BE(2).

  • Positive control
    • Rat brain cytosolic S1 fraction.
  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab198895 as a replacement.


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 15mM Sodium Azide
    Constituents: 0.01M PBS, pH 7.4
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    Purified Immunoglobulin
  • Clonality
  • Clone number
  • Myeloma
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab49849 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.02 - 0.04 µg/ml. Detects a band of approximately 300 kDa (predicted molecular weight: 191 kDa).
IP Use at an assay dependent dilution.
ICC Use at an assay dependent dilution.


  • Function
    Anchoring protein that mediates the subcellular compartmentation of protein kinase A (PKA) and protein kinase C (PKC).
  • Tissue specificity
    Expressed in endothelial cells, cultured fibroblasts and osteosarcoma, but not in platelets, leukocytes, monocytic cell lines or peripherical blood cells.
  • Sequence similarities
    Contains 3 AKAP domains.
  • Domain
    Polybasic regions located between residues 266 and 557 are involved in binding PKC.
  • Post-translational
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. May be part of the cortical cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • A kinase (PRKA) anchor protein (gravin) 12 antibody
    • A kinase Anchor Protein 12 antibody
    • A kinase anchor protein 250kDa antibody
    • A-kinase anchor protein 12 antibody
    • A-kinase anchor protein 250 kDa antibody
    • AKA12_HUMAN antibody
    • AKAP 12 antibody
    • AKAP 250 antibody
    • AKAP-12 antibody
    • Akap12 antibody
    • AKAP250 antibody
    • DKFZp686M0430 antibody
    • DKFZp686O0331 antibody
    • FLJ20945 antibody
    • FLJ97621 antibody
    • Gercelin antibody
    • Gravin antibody
    • Kinase scaffold protein gravin antibody
    • Myasthenia gravis autoantigen antibody
    • Myasthenia gravis autoantigen gravin antibody
    • OTTHUMP00000017426 antibody
    • OTTHUMP00000017427 antibody
    • Src-Suppressed C Kinase Substrate antibody
    • Srcs5 antibody
    • SSeCKS antibody
    • Tsga12 antibody
    see all


This product has been referenced in:

See all 7 Publications for this product

Customer reviews and Q&As

Thank you for your patience.

I sincerely apologize about this delay, but I have good news:

Yes, rat is a tested species and I have updated our datasheet to reflect that. We therefore guarantee this antibodyto work with rat samples. ...

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Thank you for contacting us.

I have received the following information from the lab so far:

Wedid a Blast comparison of the rat and human proteins and although the overall identity was only 68%, there are long stretches of >90%...

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Thank you for contacting us.

The lab is looking into this and will let me know if there is a reason why rat was not listed as tested species. I will get back to you when I have more information.

Thank you for your patience and unde...

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Thank you for contacting us and for alerting us to the problem you are experiencing with our Anti-AKAP12 antibody ab49849. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

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Thank you for contacting Abcam. I have looked at the western blot image that you attached and I believe that the Akap12 band is actually the large band a the top of the image, not marked by a red arrow. If you would like my opinion of which band is the...

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