Overview

  • Product nameAnti-AKAP13 antibody
    See all AKAP13 primary antibodies
  • Description
    Mouse monoclonal to AKAP13
  • Tested applicationsSuitable for: WB, IHC-P, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 1-111 of Human AKAP13

Properties

Applications

Our Abpromise guarantee covers the use of ab56917 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 308 kDa.
IHC-P Use a concentration of 1.5 - 5 µg/ml.
Sandwich ELISA Use a concentration of 0.0003 µg/ml.

Target

  • FunctionAnchors cAMP-dependent protein kinase (PKA) and acts as an adapter protein to selectively couple G alpha-13 and Rho. Augments gene activation by the estrogen receptor in an element-specific and ligand-dependent manner. Activates estrogen receptor beta by a p38 MAPK-dependent pathway. Isoform 6 stimulates exchange activity on Rho proteins in vitro, but not on CDC42, Ras or Rac and may bind calcium ions.
  • Tissue specificityIsoform 3 and isoform 6 are found in hematopoietic cells, skeletal muscle, lung, heart, estrogen-responsive reproductive tissues, including breast ductal epithelium. Also found in testis and breast cancer cell lines. Isoform 6 is not found in brain, placenta, liver, pancreas or kidney. Isoform 7 is expressed in myeloid and lymphoid lineages, a variety of epithelial tissues and skeletal muscle. Isoform 2 is predominantly found in the heart and at lower levels in the lung, placenta, kidney, pancreas, skeletal muscle and liver.
  • Sequence similaritiesContains 1 DH (DBL-homology) domain.
    Contains 1 PH domain.
    Contains 1 phorbol-ester/DAG-type zinc finger.
  • DomainBoth the DH and PH domains are required for transforming activity.
  • Cellular localizationCytoplasm; Cytoplasm. Nucleus and Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • A kinase (PRKA) anchor protein 13 antibody
    • A kinase anchor protein 13 antibody
    • A kinase anchoring protein antibody
    • A-kinase anchor protein 13 antibody
    • AKAP 13 antibody
    • AKAP Lbc antibody
    • AKAP-13 antibody
    • AKAP-Lbc antibody
    • AKAP13 antibody
    • AKP13_HUMAN antibody
    • ARHGEF13 antibody
    • Breast cancer nuclear receptor binding auxiliary protein antibody
    • Breast cancer nuclear receptor-binding auxiliary protein antibody
    • BRX antibody
    • c lbc antibody
    • FLJ11952 antibody
    • FLJ43341 antibody
    • Guanine nucleotide exchange factor Lbc antibody
    • HA 3 antibody
    • HA3 antibody
    • Ht 31 antibody
    • Ht31 antibody
    • Human thyroid anchoring protein 31 antibody
    • Human thyroid-anchoring protein 31 antibody
    • LBC antibody
    • LBC oncogene antibody
    • Lymphoid blast crisis oncogene antibody
    • Non oncogenic Rho GTPase specific GTP exchange factor antibody
    • Non-oncogenic Rho GTPase-specific GTP exchange factor antibody
    • p47 antibody
    • Protein kinase A anchoring protein 13 antibody
    • Protein kinase A-anchoring protein 13 antibody
    • PROTO LB antibody
    • PROTO LBC antibody
    see all

Anti-AKAP13 antibody images

  • Immunoperoxidase of monoclonal antibody to AKAP13 on formalin-fixed paraffin-embedded human spleen. [antibody concentration 1.5 ug/ml]
  • Anti-AKAP13 antibody (ab56917) + HeLa

    Predicted band size : 308 kDa

References for Anti-AKAP13 antibody (ab56917)

This product has been referenced in:
  • Ahmad F  et al. Regulation of sarcoplasmic reticulum Ca2+ ATPase 2 (SERCA2) activity by phosphodiesterase 3A (PDE3A) in human myocardium: phosphorylation-dependent interaction of PDE3A1 with SERCA2. J Biol Chem 290:6763-76 (2015). Read more (PubMed: 25593322) »

See 1 Publication for this product

Product Wall

Thank you for contacting us about this unusual AKAP13 western blot result. The originator of the antibody believes that it is recognizing isoform 6 of AKAP13 in the K562 lysate. Isoforms of AKAP13 are tissue specific, and that is apparently the case fo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"