Overview

  • Product nameAnti-AKAP13 antibody
    See all AKAP13 primary antibodies
  • Description
    Rabbit polyclonal to AKAP13
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region between residue 2754 and 2804 of Human AKAP13 (NP_009131.2)

  • Positive control
    • HeLa whole cell lysate
  • General notesImmunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab99377 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 308 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • FunctionAnchors cAMP-dependent protein kinase (PKA) and acts as an adapter protein to selectively couple G alpha-13 and Rho. Augments gene activation by the estrogen receptor in an element-specific and ligand-dependent manner. Activates estrogen receptor beta by a p38 MAPK-dependent pathway. Isoform 6 stimulates exchange activity on Rho proteins in vitro, but not on CDC42, Ras or Rac and may bind calcium ions.
  • Tissue specificityIsoform 3 and isoform 6 are found in hematopoietic cells, skeletal muscle, lung, heart, estrogen-responsive reproductive tissues, including breast ductal epithelium. Also found in testis and breast cancer cell lines. Isoform 6 is not found in brain, placenta, liver, pancreas or kidney. Isoform 7 is expressed in myeloid and lymphoid lineages, a variety of epithelial tissues and skeletal muscle. Isoform 2 is predominantly found in the heart and at lower levels in the lung, placenta, kidney, pancreas, skeletal muscle and liver.
  • Sequence similaritiesContains 1 DH (DBL-homology) domain.
    Contains 1 PH domain.
    Contains 1 phorbol-ester/DAG-type zinc finger.
  • DomainBoth the DH and PH domains are required for transforming activity.
  • Cellular localizationCytoplasm; Cytoplasm. Nucleus and Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • A kinase (PRKA) anchor protein 13 antibody
    • A kinase anchor protein 13 antibody
    • A kinase anchoring protein antibody
    • A-kinase anchor protein 13 antibody
    • AKAP 13 antibody
    • AKAP Lbc antibody
    • AKAP-13 antibody
    • AKAP-Lbc antibody
    • AKAP13 antibody
    • AKP13_HUMAN antibody
    • ARHGEF13 antibody
    • Breast cancer nuclear receptor binding auxiliary protein antibody
    • Breast cancer nuclear receptor-binding auxiliary protein antibody
    • BRX antibody
    • c lbc antibody
    • FLJ11952 antibody
    • FLJ43341 antibody
    • Guanine nucleotide exchange factor Lbc antibody
    • HA 3 antibody
    • HA3 antibody
    • Ht 31 antibody
    • Ht31 antibody
    • Human thyroid anchoring protein 31 antibody
    • Human thyroid-anchoring protein 31 antibody
    • LBC antibody
    • LBC oncogene antibody
    • Lymphoid blast crisis oncogene antibody
    • Non oncogenic Rho GTPase specific GTP exchange factor antibody
    • Non-oncogenic Rho GTPase-specific GTP exchange factor antibody
    • p47 antibody
    • Protein kinase A anchoring protein 13 antibody
    • Protein kinase A-anchoring protein 13 antibody
    • PROTO LB antibody
    • PROTO LBC antibody
    see all

Anti-AKAP13 antibody images

  • All lanes : Anti-AKAP13 antibody (ab99377) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg


    Predicted band size : 308 kDa


    Exposure time : 10 seconds
  • Lane 1 : Detection of Human AKAP13 by Immunoprecipitation from HeLa Whole cell lysate (1 mg for IP, 20% of IP loaded) using 3µg/mg lysate ab99377 lane 2 : Control IgG

References for Anti-AKAP13 antibody (ab99377)

ab99377 has not yet been referenced specifically in any publications.

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