Recombinant
RabMAb

Anti-AKAP14 antibody [EPR12955] - C-terminal (ab180160)

Overview

  • Product name
    Anti-AKAP14 antibody [EPR12955] - C-terminal
  • Description
    Rabbit monoclonal [EPR12955] to AKAP14 - C-terminal
  • Tested applications
    Suitable for: WB, IP, ICC/IFmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human AKAP14 aa 100 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q86UN6-1

  • Positive control
    • Jurkat, HUVEC, NCI-H460, A549 and Human fetal kidney lysates; A549 cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR12955
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab180160 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 23 kDa.
IP 1/10 - 1/100.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.
  • Target

    Images

    • All lanes : Anti-AKAP14 antibody [EPR12955] - C-terminal (ab180160) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : HUVEC cell lysate
      Lane 3 : NCI-H460 cell lysate
      Lane 4 : A549 cell lysate
      Lane 5 : Human fetal kidney lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution
      Developed using the ECL technique

      Predicted band size : 23 kDa
    • Immunofluorescence analysis of A549 cells labeling AKAP14 with ab180160 at a 1/100 dilution.

    • Western blot analysis on Immunoprecipitation pellet from either 1) Jurkat cell lysate, or 2) 1xPBS (negative control); showing AKAP14, using ab180160 at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    References

    ab180160 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab180160.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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